Molecular characterisation and expression analysis of a fish-egg lectin in rock bream, and its response to bacterial or viral infection

被引:17
|
作者
Kim, Bong-Seok [2 ]
Nam, Bo-Hye [2 ]
Kim, Ju-Won [1 ]
Park, Hyoung-Jun [1 ]
Song, Jeong-Hun [3 ]
Park, Chan-Il [1 ]
机构
[1] Gyeongsang Natl Univ, Inst Marine Ind, Dept Marine Biol & Aquaculture, Coll Marine Sci, Tongyeong 650160, South Korea
[2] Natl Fisheries Res & Dev Inst, Biotechnol Res Div, Pusan 619902, South Korea
[3] Pukyong Natl Univ, Coll Fisheries Sci, Pusan 608737, South Korea
关键词
Fertilized egg; Fish-egg lectin; Ontogeny; Rock bream; SALMO-GAIRDNERI RICHARDSON; TROUT ONCORHYNCHUS-MYKISS; RHAMNOSE-BINDING LECTINS; RAINBOW-TROUT; SEQUENCE; ONTOGENY; PROTEIN; CELLS; PCR;
D O I
10.1016/j.fsi.2011.10.024
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
A fish-egg lectin, RbFEL, was identified from rock bream (Oplegnathus fasciatus) and its expression analysed. In both vertebrates and invertebrates, carbohydrate-binding proteins (lectins) play an important role in innate immunity against microbial invasion. Here, we report the cloning of a fish-egg lectin from rock bream using a combination of expression sequence tag (EST) analyses. The full-length cDNA of RbFEL is composed of 1512-bp with a 780-bp ORF that encodes 259 amino acid residues. The deduced polypeptide exhibits six conserved residues of the FEL family. All cysteine positions in each domain were completely conserved. Reverse transcription quantitative real-time PCR analysis of the tissues revealed that RbFEL mRNA was abundantly expressed in liver, moderately expressed in head kidney and rarely expressed in other tissues. Expression analyses of time series sampled fertilised eggs showed that expression gradually increased 1, 3, 12, 24 and 36 h after fertilisation. In addition, RbFEL expression was significantly up-regulated in rock bream challenged with Edwardsiella tarda, Streptococcus iniae and red sea bream iridovirus (RSIV). These results suggest that RbFEL is a member of the egg-lectin family and is involved in the innate immune response in rock bream. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1201 / 1207
页数:7
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