Identifying insertion mutations by whole-genome sequencing

被引：11
|
作者：
Smith, Harold E. [1 ]
机构：
[1] NIDDKD, NIH, Bethesda, MD 20892 USA
来源：
BIOTECHNIQUES | 2011年 / 50卷 / 02期
基金：
美国国家卫生研究院;
关键词：
Next-generation sequencing; whole-genome resequencing; mutation identification; insertion mapping; ESCHERICHIA-COLI;
D O I：
10.2144/000113600
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Insertion mutagenesis via mobile genetic element is a common technique for the analysis of gene function in model organisms. Next-generation sequencing offers an attractive approach for localizing the site of insertion, but alignment-based mapping of mobile genetic elements is challenging. A computational method for identifying insertion sites is reported herein. The technique was validated by mapping transposons in both bacterial and nematode species. The approach should be extensible to other systems that employ mobile genetic elements to generate mutations.
引用
收藏
页码：96 / 97
页数：2
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