A Strategy for Direct Mapping and Identification of Mutations by Whole-Genome Sequencing

被引：114

作者：

Zuryn, Steven ^{[1
]}

Le Gras, Stephanie ^{[1
]}

Jamet, Karine ^{[1
]}

Jarriault, Sophie ^{[1
]}

机构：

[1] Univ Strasbourg, CNRS, UMR 1704, Inst Genet & Biol Mol & Cellulaire,INSERM,U964, F-67404 Strasbourg, France

来源：

GENETICS | 2010年 / 186卷 / 01期

关键词：

CAENORHABDITIS-ELEGANS; C-ELEGANS;

D O I：

10.1534/genetics.110.119230

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Mutant screens have proven powerful for genetic dissection of a myriad of biological processes, but subsequent identification and isolation of the causative mutations are usually complex and time consuming. We have made the process easier by establishing a novel strategy that employs whole-genome sequencing to simultaneously map and identify mutations without the need for any prior genetic mapping.

引用

页码：427 / 430

页数：4

共 50 条

[1] Mutation mapping and identification by whole-genome sequencing
Leshchiner, Ignaty
Alexa, Kristen
Kelsey, Peter
Adzhubei, Ivan
Austin-Tse, Christina A.
Cooney, Jeffrey D.
Anderson, Heidi
King, Matthew J.
Stottmann, Rolf W.
Garnaas, Maija K.
Ha, Seungshin
Drummond, Iain A.
Paw, Barry H.
North, Trista E.
Beier, David R.
Goessling, Wolfram
Sunyaev, Shamil R.
[J]. GENOME RESEARCH, 2012, 22 (08) : 1541 - 1548
[2] Mapping Challenging Mutations by Whole-Genome Sequencing Aimee
Smith, Harold E.
Fabritius, Amy S.
Jaramillo-Lambert, Aimee
Golden, Andy
[J]. G3-GENES GENOMES GENETICS, 2016, 6 (05): : 1297 - 1304
[3] Using whole-genome sequencing to discover mutations
Laffman-Johnson, Elise
[J]. CLINICAL PHARMACOLOGY & THERAPEUTICS, 2009, 85 (02) : 118 - 118
[4] Identifying insertion mutations by whole-genome sequencing
Smith, Harold E.
[J]. BIOTECHNIQUES, 2011, 50 (02) : 96 - 97
[5] Whole-genome sequencing identifies Mendelian mutations
Meera Swami
[J]. Nature Reviews Genetics, 2010, 11 : 313 - 313
[6] Identification of EMS-Induced Mutations in Drosophila melanogaster by Whole-Genome Sequencing
Blumenstiel, Justin P.
Noll, Aaron C.
Griffiths, Jennifer A.
Perera, Anoja G.
Walton, Kendra N.
Gilliland, William D.
Hawley, R. Scott
Staehling-Hampton, Karen
[J]. GENETICS, 2009, 182 (01) : 25 - 32
[7] Efficient Mapping and Cloning of Mutations in Zebrafish by Low-Coverage Whole-Genome Sequencing
Bowen, Margot E.
Henke, Katrin
Siegfried, Kellee R.
Warman, Matthew L.
Harris, Matthew P.
[J]. GENETICS, 2012, 190 (03) : 1017 - U229
[8] Whole-genome sequencing
Morris, Huw R.
Houlden, Henry
Polke, James
[J]. PRACTICAL NEUROLOGY, 2021, 21 (04) : 322 - +
[9] Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing
Obholzer, Nikolaus
Swinburne, Ian A.
Schwab, Evan
Nechiporuk, Alex V.
Nicolson, Teresa
Megason, Sean G.
[J]. DEVELOPMENT, 2012, 139 (22): : 4280 - 4290
[10] Mapping epigenetic mutations in fission yeast using whole-genome next-generation sequencing
Irvine, Danielle V.
Goto, Derek B.
Vaughn, Matthew W.
Nakaseko, Yukinobu
McCombie, W. Richard
Yanagida, Mitsuhiro
Martienssen, Rob
[J]. GENOME RESEARCH, 2009, 19 (06) : 1077 - 1083

← 1 2 3 4 5 →