Enhanced throughput for DNA sequencing by capillary array electrophoresis with a gradient of electric field strength

被引：5
|
作者：
Yoshida, C [1 ]
Endo, Y [1 ]
Baba, Y [1 ]
机构：
[1] Univ Tokushima, Fac Pharmaceut Sci, Dept Med Chem, Tokushima 7708505, Japan
来源：
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2001年 / 13卷 / 01期
关键词：
DNA sequencing; SNPs; gradient of electric field strength; capillary temperature;
D O I：
10.1016/S0928-0987(00)00213-X
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The effect of electric field gradients are examined on the speed, selectivity, read length, and accuracy for DNA sequencing using capillary array electrophoresis. Modified electric field gradients was realized to read over 800 bases within 140 min. The method developed is effectively applicable to single nucleotide polymorphism analysis for genomic drug discovery and pharmacogenomics. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：99 / 103
页数：5
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