TRANSCRIPT ANALYSIS OF STEM CELLS

被引：19
|
作者：
Nairn, Alison V. [1 ,2 ]
dela Rosa, Mitche [1 ,2 ]
Moremen, Kelley W. [1 ,2 ]
机构：
[1] Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA
[2] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
来源：
METHODS IN ENZYMOLOGY, VOL 479: FUNCTIONAL GLYCOMICS | 2010年 / 479卷
关键词：
HEPARAN-SULFATE PROTEOGLYCANS; BIOLOGICAL ROLES; PCR DATA; EXPRESSION; CERAMIDE; GENES; MOUSE; GLYCOSYLATION; APOPTOSIS; DATABASE;
D O I：
10.1016/S0076-6879(10)79004-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Quantitative real-time polyrnerase chain reaction (qRT-PCR) is a flexible and scalable method for analyzing transcript abundance that can be used at a single gene or high-throughput (> 100 genes) level. Information obtained from this technique can be used as an indicator of potential regulation of glycosylation at the transcript Level when combined with glycan structural or protein abundance data. This chapter describes detailed methods to design and perform qRT-PCR analyses and provides examples of information that can be obtained from the technique.
引用
收藏
页码：73 / 91
页数：19
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