Mast cell degranulation via MRGPRX2 by isolated human albumin fragments

被引:20
|
作者
Karhu, T. [1 ,2 ]
Akiyama, K. [3 ]
Vuolteenaho, O. [1 ,4 ]
Bergmann, U. [2 ,5 ]
Naito, T. [3 ,6 ]
Tatemoto, K. [1 ,2 ]
Herzig, K. -H. [1 ,2 ,7 ,8 ,9 ]
机构
[1] Univ Oulu, Res Unit Biomed, POB 5000, FIN-90014 Oulu, Finland
[2] Univ Oulu, Bioctr Oulu, POB 5000, FIN-90014 Oulu, Finland
[3] Grad Univ, Okinawa Inst Sci & Technol, Kunigami, Okinawa, Japan
[4] Nordlab Oulu, Oulu, Finland
[5] Univ Oulu, Fac Biochem & Mol Med, Oulu, Finland
[6] Kanagawa Univ, Res Inst Nat Drug Leads, Hiratsuka, Kanagawa, Japan
[7] Poznan Univ Med Sci, Dept Gastroenterol & Metab, Poznan, Poland
[8] Med Res Ctr, Oulu, Finland
[9] Oulu Univ Hosp, Oulu, Finland
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2017年 / 1861卷 / 11期
关键词
Albumin; GPCR; Inflammation; Mast cell; MRGPRX2; GENE X2; ACTIVATION; PEPTIDE; EXPRESSION; FAMILY; MRGX2;
D O I
10.1016/j.bbagen.2017.08.013
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background-Mast cells are important modulators of the human immune system via their release of several inflammatory mediators and proteases. The release can be activated by different pathways: the classical immunoglobulin E-dependent pathway and by the non-immunological immunoglobulin E-independent pathway. MAS-related G protein-coupled receptor X2 (MRGPRX2) is expressed in mast cells and it is one of the endogenous receptor responsible for the IgE-independent activation of human mast cell. The MRGPRX2 is classified as orphan receptor and unlike most GPCRs, the MRGPRX2 recognizes a wide range of basic molecules. Thus, there still might be several unknown ligands for the receptor. Methods: MRGPRX2 activating peptides were isolated from human plasma using consecutive HPLC purification steps. The isolation process was monitored with MRGPRX2 transfected HEK 293 cells. The isolated peptides were sequenced by MS and synthetized. The synthetic peptides were used to determine degranulation of the human LAD 2 mast cell line by measuring beta-hexosaminidase release. Results: Three endogenous MRGPRX2 activating peptides were isolated from human plasma. These peptides are identified as fragments of albumin. The isolated fragments activate MRGPRX2 and degranulate MRGPRX2 expressing LAD 2 cells in dose-dependent manner. Conclusions: The isolated basic peptides generated from human albumin are able to degranulate mast cells via the MRGPRX2.
引用
收藏
页码:2530 / 2534
页数:5
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