Objective: To explore the hepatoprotective and anti oxidant activities of the methanolic leaf extract of Bridelia micrantha (B. micrantha) on paracetamol induced liver damage in Wistar rats. Parameters were measured including alanine aminotransaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin and total protein. The anti oxidant effects were studied using the 1, 1-Diphenynl-2 Picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (TRAP) assay methods. B. micrantha extract decreased the level of AST in the rats given PCM from (129.47 +/- 0.92I) IU/L to (57.78 +/- 1.71) IU/L (P<0.05). This was lower than the value for Silymarin which was (59.92 +/- 1.41) IU/L. ALT concentration was reduced from (150.18 +/- 2.23) IU/L to (79.10 +/- 2.01) IU/L (p<0.05). ALP was reduced from (49.86 +/- 0.85) IU/L to (29.64 +/- 1.53) IU/L (P<0.05). Total bilirubin was reduced from (2.14 +/- 0.10 mg/dL) to (0.18 +/- 0.07) mg/dL (P<0.05) while total protein was increased from (4.26 +/- 0.30) mg/dL to (6.20 +/- 0.19) mg/dL (P<0.05). Concentrations ranging from 10 - 400 mu g/mL of B. micrantha were assayed for antioxidant activities. The DPPH assay showed 98% antioxidant activity at concentration of 400 1,1 mu g/mL. The FRAP values were 0.016. 0.39, 0.455, 0.601 and 1.382 mu M at 10, 50. 100. 200 and 400 mu g/mL respectively. Conclusion: Results suggest that B. micrantha has hepatoprotective and anti oxidant potentials. However, further work involving fractionation needs to done to isolate the active compound responsible for the hepatoprotective activity.
机构:
Annamalai Univ, Fac Sci, Dept Biochem, Annamalainagar 608002, Tamil Nadu, IndiaAnnamalai Univ, Fac Sci, Dept Biochem, Annamalainagar 608002, Tamil Nadu, India
Kamalakkannan, N
Prince, PSM
论文数: 0引用数: 0
h-index: 0
机构:
Annamalai Univ, Fac Sci, Dept Biochem, Annamalainagar 608002, Tamil Nadu, IndiaAnnamalai Univ, Fac Sci, Dept Biochem, Annamalainagar 608002, Tamil Nadu, India
机构:
Minnan Normal Univ, Coll Biol Sci & Technol, Zhangzhou 363000, Peoples R ChinaMinnan Normal Univ, Coll Biol Sci & Technol, Zhangzhou 363000, Peoples R China
Zheng, Yi Mei
Shen, James Zheng
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R ChinaMinnan Normal Univ, Coll Biol Sci & Technol, Zhangzhou 363000, Peoples R China
Shen, James Zheng
Wang, Yan
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R ChinaMinnan Normal Univ, Coll Biol Sci & Technol, Zhangzhou 363000, Peoples R China
Wang, Yan
Lu, Amy Xiaoxu
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R ChinaMinnan Normal Univ, Coll Biol Sci & Technol, Zhangzhou 363000, Peoples R China
Lu, Amy Xiaoxu
Ho, Wing Shing
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R ChinaMinnan Normal Univ, Coll Biol Sci & Technol, Zhangzhou 363000, Peoples R China
机构:
Hubei Univ Chinese Med, Educ Minist Tradit Chinese Med Resource & Compoun, Key Lab, Wuhan 430065, Hubei, Peoples R China
Hubei Univ Med, Coll Pharm, Shiyan 442000, Hubei, Peoples R ChinaHubei Univ Chinese Med, Educ Minist Tradit Chinese Med Resource & Compoun, Key Lab, Wuhan 430065, Hubei, Peoples R China
Wang, Jia-Zhi
Huang, Bi-Sheng
论文数: 0引用数: 0
h-index: 0
机构:
Hubei Univ Chinese Med, Educ Minist Tradit Chinese Med Resource & Compoun, Key Lab, Wuhan 430065, Hubei, Peoples R ChinaHubei Univ Chinese Med, Educ Minist Tradit Chinese Med Resource & Compoun, Key Lab, Wuhan 430065, Hubei, Peoples R China
Huang, Bi-Sheng
Cao, Yan
论文数: 0引用数: 0
h-index: 0
机构:
Hubei Univ Chinese Med, Educ Minist Tradit Chinese Med Resource & Compoun, Key Lab, Wuhan 430065, Hubei, Peoples R ChinaHubei Univ Chinese Med, Educ Minist Tradit Chinese Med Resource & Compoun, Key Lab, Wuhan 430065, Hubei, Peoples R China
Cao, Yan
Chen, Ke-Li
论文数: 0引用数: 0
h-index: 0
机构:
Hubei Univ Chinese Med, Educ Minist Tradit Chinese Med Resource & Compoun, Key Lab, Wuhan 430065, Hubei, Peoples R ChinaHubei Univ Chinese Med, Educ Minist Tradit Chinese Med Resource & Compoun, Key Lab, Wuhan 430065, Hubei, Peoples R China
Chen, Ke-Li
Li, Juan
论文数: 0引用数: 0
h-index: 0
机构:
Hubei Univ Chinese Med, Educ Minist Tradit Chinese Med Resource & Compoun, Key Lab, Wuhan 430065, Hubei, Peoples R ChinaHubei Univ Chinese Med, Educ Minist Tradit Chinese Med Resource & Compoun, Key Lab, Wuhan 430065, Hubei, Peoples R China