Development of recombinant canine adenovirus type-2 expressing the Gn glycoprotein of Seoul virus

被引：2

作者：

Yuan, Ziguo ^{[1
,2
]}

Zhang, Xiuxiang ^{[2
]}

Zhang, Shoufeng ^{[1
]}

Liu, Ye ^{[1
]}

Gao, Shengyan ^{[3
]}

Zhang, Fei ^{[1
]}

Xu, Huijuan ^{[2
]}

Wang, Xiaohu ^{[2
]}

Hu, Rongliang ^{[1
]}

机构：

[1] Acad Mil Med Sci, Vet Inst, Lab Epidemiol, Changchun 130062, Peoples R China

[2] Jilin Univ, Div Agr, Coll Anim Sci & Vet Med, Changchun 130062, Peoples R China

[3] Jilin Agr Univ, Coll Life Sci, Changchun 130062, Peoples R China

来源：

BIOLOGICALS | 2008年 / 36卷 / 03期

关键词：

hantavirus; Seoul virus; canine adenovirus type-2; Gn;

D O I：

10.1016/j.biologicals.2007.10.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Seoul virus glycoprotein Gn is a major structural protein and candidate antigen of hantavirus that induces a highly immunogenic response for hantavirus vaccine. In this study, a replication-competent recombinant canine adenovirus type-2 expressing Gn was constructed by the in vitro ligation method. The Gn expression cassette, including the human cytomegalovirus (hCMV) promoter/enhancer and the SV40 early mRNA polyadenylation signal, was cloned into the SspI site of the E3 region which is not essential for proliferation of CAV-2. Expression of Gn was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. (C) 2007 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.

引用

页码：162 / 167

页数：6

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