Optimization of culture conditions for bacterial cellulose production from Gluconacetobacter hansenii UAC09

被引：47
|
作者：
Rani, Mahadevaswamy Usha [1 ]
Appaiah, Anu [1 ]
机构：
[1] Cent Food Technol Res Inst, Dept Food Microbiol, Mysore 570020, Karnataka, India
来源：
ANNALS OF MICROBIOLOGY | 2011年 / 61卷 / 04期
关键词：
Bacterial cellulose; Fermentation; Optimization; Gluconacetobacter hansenii; MICROBIAL CELLULOSE; CARBON;
D O I：
10.1007/s13213-011-0196-7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Fermentation conditions for the production of bacterial cellulose (BC) by a newly isolated Gluconacetobacter hansenii UAC09 were optimized. The effect of various carbon and nitrogen sources on production of BC was determined. The addition of ethanol (1.5%) and acetic acid (1.0%) enhanced BC production by more than three times compared to standard Hestrin and Schramm medium. The strain was able to produce maximum BC (7.40 g 1(-1)) with 4% glucose, 8% corn steep liquor (CSL) at 27 +/- 1 degrees C and a pH of 5.5 under stationary conditions. The BC films produced under various conditions were tested for their structural similarity using Fourier transform-infra red analysis and were found to be similar to each other irrespective of medium composition. Scanning electron microscopy images of BC revealed a porous structure with extensively interwoven microfibrils.
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页码：781 / 787
页数：7
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