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Long-term protection from SARS coronavirus infection conferred by a single immunization with an attenuated VSV-based vaccine
被引:132
|作者:
Kapadia, SU
Rose, JK
Lamirande, E
Vogel, L
Subbarao, K
Roberts, A
机构:
[1] Yale Univ, Sch Med, Dept Pathol, New Haven, CT 06510 USA
[2] Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06510 USA
[3] NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA
来源:
关键词:
intranasal;
neutralizing antibodies;
protective immunity;
spike;
D O I:
10.1016/j.virol.2005.06.016
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Although the recent SARS coronavirus (SARS-CoV) that appeared in 2002 has now been contained, the possibility of re-emergence of SARS-CoV remains. Due to the threat of re-emergence, the overall fatality rate of similar to 10%, and the rapid dispersion of the virus via international travel, viable vaccine candidates providing protection from SARS are clearly needed. We developed an attenuated VSV recombinant (VSV-S) expressing the SARS coronavirus (SARS-CoV) spike (S) protein. In cells infected with this recombinant, S protein was synthesized, glycosylated at approximately 17 Asn residues, and transported via the Golgi to the cell surface. Mice vaccinated with VSV-S developed SARS-neutralizing antibody and were able to control a challenge with SARS-CoV performed at I month or 4 months after a single vaccination. We also demonstrated, by passive antibody transfer, that the antibody response induced by the vaccine was sufficient for controlling SARS-CoV infection. A VSV-vectored SARS vaccine could have significant advantages over other SARS vaccine candidates described to date. (c) 2005 Published by Elsevier Inc.
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页码:174 / 182
页数:9
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