Detection of apoptosis in vivo using antibodies against caspase-induced neo-epitopes

被引：43
|
作者：
Jakob, Sabine [1 ]
Corazza, Nadia [1 ]
Diamantis, Eva [2 ]
Kappeler, Andreas [1 ]
Brunner, Thomas [1 ]
机构：
[1] Univ Bern, Inst Pathol, Div Immunopathol, CH-3010 Bern, Switzerland
[2] Univ Bern, Inst Pathol, Gen Clin Histopathol, CH-3010 Bern, Switzerland
来源：
METHODS | 2008年 / 44卷 / 03期
关键词：
apoptosis; liver; caspase; lamin A; Fas/CD95; immunohistochemistry; paraffin sections; histology; antigen retrieval;
D O I：
10.1016/j.ymeth.2007.11.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Cell death induction by apoptosis is an important process in the maintenance of tissue homeostasis as well as tissue destruction during various pathological processes. Consequently, detection of apoptotic cells in situ represents an important technique to assess the extent and impact of cell death in the respective tissue. While scoring of apoptosis by histological assessment of apoptotic cells is still a widely used method, it is likely biased by sensitivity problems and observed-based variations. The availability of caspase-mediated neo-epitope-specific antibodies offers new tools for the detection of apoptosis in situ. Here, we discuss the use of immunohistochemical detection of cleaved caspase 3 and lamin A for the assessment of apoptotic cells in paraffin-embedded liver tissue. Furthermore, we evaluate the effect of tissue pretreatment and antigen retrieval on the sensitivity of apoptosis detection, background staining and maintenance of tissue morphology. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：255 / 261
页数：7
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