In vitro Evaluation of the Genotoxic and Cytotoxic Effects of Artesunate, An Antimalarial Drug, in Human Lymphocytes

被引：18
|
作者：
Mota, Tatiane C. [1 ]
Cardoso, Plinio C. S. [1 ]
Gomes, Lorena M. [1 ]
Vieira, Priscilla C. M. [1 ]
Correa, Regianne M. S. [1 ]
Santana, Priscila Di Paula B. [2 ]
Miranda, Moyses S. [2 ]
Burbano, Rommel M. R. [1 ]
Bahia, Marcelo O. [1 ]
机构：
[1] Fed Univ Para, Inst Ciencias Biol, Lab Citogenet Humana, BR-66075110 Belem, Para, Brazil
[2] Fed Univ Para, Inst Ciencias Biol, Lab Fecundacao Vitro, BR-66075110 Belem, Para, Brazil
来源：
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS | 2011年 / 52卷 / 07期
关键词：
artesunate; genotoxicity; apoptosis; micronucleus; comet assay; SESQUITERPENE LACTONE; DNA-DAMAGE; TUMOR-CELLS; ARTEMISININ; ASSAY; REPAIR; DEATH; VIVO;
D O I：
10.1002/em.20659
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Artesunate is one of the main antimalarial drugs used in several countries. It is a semisynthetic compound derived from artemisinin, a substance extracted from the Chinese plant, Artemisia annua L. Despite the widespread use of artesunate as an antimalarial drug, there is a lack of data regarding its genotoxic effects in human lymphocytes. Therefore, in this study, we used the comet assay and micronucleus test to evaluate the possible genotoxic effects of artesunate in cultured human lymphocytes. In addition, cell death by necrosis and apoptosis was also assessed. Cells exposed to different concentrations of artesunate showed a significant concentration-dependent increase (P < 0.05) in DNA damage index and micronuclei frequency. A significant increase in the frequency of apoptotic and necrotic cells was also observed. Our results showed that artesunate is a genotoxic and cytotoxic compound in cultured human lymphocytes. Environ. Mol. Mutagen. 52:590-594, 2011. (C) 2011 Wiley-Liss, Inc.
引用
收藏
页码：590 / 594
页数：5
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