UPLC-ESI-MS/MS Based Characterization of Active Flavonoids from Apocynum spp. and Anti-Bacteria Assay

被引：10
|
作者：
Gao, Gang [1 ]
Liu, Ning [1 ]
Yu, Chunming [1 ]
Chen, Ping [1 ]
Chen, Jikang [1 ]
Chen, Kunmei [1 ]
Wang, Xiaofei [1 ]
Liu, Bin [2 ]
Zhu, Aiguo [1 ]
机构：
[1] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Peoples R China
[2] Hunan Univ, Coll Biol, Changsha 410082, Peoples R China
来源：
ANTIOXIDANTS | 2021年 / 10卷 / 12期
关键词：
Apocynum spp; flavonoids; antioxidant; antimicrobial; wound healing; ANTIOXIDANT CAPACITY; OXIDATIVE STRESS; VENETUM; IDENTIFICATION; CONSTITUENTS; MEDICINE; EXTRACTS; LEAVES; ACIDS; RATS;
D O I：
10.3390/antiox10121901
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In the current study, the active flavonoids from Apocynum venetum and Apocynum hendersonii leaf were efficiently characterized using UPLC-ESI-MS/MS, and yielding the highest content of 15.35 mg/g (A. venetum) and 13.28 mg/g (A. hendersonii) respectively. The antioxidant assay in vitro showed that the isolated flavonoid ingredient groups exhibited free radical scavenging activities to DPPH, ABTS and linoleic acid. The antimicrobial assay revealed the isolated flavonoid ingredient from both A. venetum and A. hendorsonii have exerted anti-MRSA and anti-P. aeruginosa effect through disrupting cell integrity and declining ATP. In vivo assay demonstrated that these flavonoid ingredients effectively accelerated MRSA-infected and P. aeruginosa-infected Balb/c mice wound healing. In summary, these results showed that the characterized flavonoid ingredients exhibited great potential as natural antioxidant and antimicrobial agents, and shed light into future potential applications of Apocynum spp.
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页数：14
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