Quantitation of 25-hydroxyvitamin D2 and D3 in serum and plasma by LCMS/MS

被引：9
|
作者：
Lee, Dayong [1 ]
Garrett, Timothy J. [1 ]
Goldberger, Bruce A. [1 ]
Bazydlo, Lindsay A. L. [1 ,2 ]
机构：
[1] Univ Florida, Coll Med, Dept Pathol Immunol & Lab Med, UF Hlth Pathol Labs, Gainesville, FL 32611 USA
[2] Univ Virginia, Charlottesville, VA 22908 USA
来源：
BIOANALYSIS | 2015年 / 7卷 / 02期
关键词：
TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; VITAMIN-D DEFICIENCY; ASSAY; RISK; METABOLITES; EXTRACTION; THROUGHPUT; CANCER; D2;
D O I：
10.4155/bio.14.229
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Objective: We developed and validated an analytical method for quantifying 25-hydroxyvitamin D-2 (25OHD(2)) and 25-hydroxyvitamin D-3 (25OHD(3)) in serum and plasma. Methods: Samples, pretreated with zinc sulfate and methanol, were extracted with hexane. Separation was achieved via UHPLC and 25OHD quantification was accomplished by a triple quadrupole mass spectrometer. Results: Imprecision was 3.6-15.1%CV and bias 88.0-126.0%. Extraction efficiency was 76.5-110.5%, whereas the matrix effect ranged from -46.7 to -32.0%. The method was applied to authentic specimens. The results showed no significant difference between serum and plasma; strong correlation with paired values from an external laboratory; and analyte stability for 15 days. Conclusion: This method provides reliable and accurate measurement of 25OHD for use in clinical practice.
引用
收藏
页码：167 / 178
页数：12
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