Crystal structure of zinc-finger domain of Nanos and its functional implications

被引：29

作者：

Hashimoto, Hiroshi ^{[1
]}

Hara, Kodai ^{[1
]}

Hishiki, Asami ^{[1
]}

Kawaguchi, Shigeta ^{[1
]}

Shichijo, Naoki ^{[1
]}

Nakamura, Keishi ^{[2
]}

Unzai, Satoru ^{[1
]}

Tamaru, Yutaka ^{[2
]}

Shimizu, Toshiyuki ^{[3
]}

Sato, Mamoru ^{[1
]}

机构：

[1] Yokohama City Univ, Grad Sch Nanobiosci, Tsurumi Ku, Kanagawa 2300045, Japan

[2] Mie Univ, Grad Sch Bioresources, Tsu, Mie 5148507, Japan

[3] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

EMBO REPORTS | 2010年 / 11卷 / 11期

关键词：

crystal structure; germ cell; Nanos; RNA-binding protein; translational regulation; GERMLINE STEM-CELLS; DNA-BINDING DOMAIN; X-RAY-DIFFRACTION; MESSENGER-RNA; TRANSLATIONAL CONTROL; NUCLEOCAPSID PROTEIN; DROSOPHILA EMBRYOS; ZEBRAFISH NANOS; PUMILIO; RECOGNITION;

D O I：

10.1038/embor.2010.155

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nanos is an RNA-binding protein that is involved in the development and maintenance of germ cells. In combination with Pumilio, Nanos binds to the 3' untranslated region of a messenger RNA and represses its translation. Nanos has two conserved Cys-Cys-His-Cys zinc-finger motifs that are indispensable for its function. In this study, we have determined the crystal structure of the zinc-finger domain of zebrafish Nanos, for the first time revealing that Nanos adopts a novel zinc-finger structure. In addition, Nanos has a conserved basic surface that is directly involved in RNA binding. Our results provide the structural basis for further studies to clarify Nanos function.

引用

下载

页码：848 / 853

页数：6

共 50 条

[31] Mutations in the zinc-finger domain of the human hairless gene underlie congenital atrichia
Ahmad, W
Nomura, K
Kozlowska, U
Kozlowska, A
Hashimoto, I
Jablonska, S
Christiano, AM
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1999, 112 (04) : 640 - 640
[32] Structure of N-terminal domain of ZAP indicates how a zinc-finger protein recognizes complex RNA
Chen, Shoudeng
Xu, Yihui
Zhang, Kuo
Wang, Xinlu
Sun, Jian
Gao, Guangxia
Liu, Yingfang
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2012, 19 (04) : 430 - 435
[33] Protein kinase Ccurly epsilon is localized to the Golgi via its zinc-finger domain and modulates Golgi function
Lehel, C.
Olah, Z.
Jakab, G.
Anderson, W. B.
Proceedings of the National Academy of Sciences of the United States of America, 1995, 92 (05):
[34] MASS-SPECTROMETRIC ANALYSIS OF A NATIVE ZINC-FINGER STRUCTURE - THE GLUCOCORTICOID RECEPTOR DNA-BINDING DOMAIN
WITKOWSKA, HE
SHACKLETON, CHL
DAHLMANWRIGHT, K
KIM, JY
GUSTAFSSON, JA
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (12) : 3319 - 3324
[35] Identification and Structure-Activity Relationship of HDAC6 Zinc-Finger Ubiquitin Binding Domain Inhibitors
de Freitas, Renato Ferreira
Harding, Rachel J.
Franzoni, Ivan
Ravichandran, Mani
Mann, Mandeep K.
Ouyang, Hui
Lautens, Mark
Santhakumar, Vijayaratnam
Arrowsmith, Cheryl H.
Schapira, Matthieu
JOURNAL OF MEDICINAL CHEMISTRY, 2018, 61 (10) : 4517 - 4527
[36] METAL REPLACEMENT IN ZINC-FINGER AND ITS EFFECT ON DNA-BINDING
PREDKI, PF
SARKAR, B
ENVIRONMENTAL HEALTH PERSPECTIVES, 1994, 102 : 195 - 198
[37] Overcharging of the Zinc Ion in the Structure of the Zinc-Finger Protein Is Needed for DNA Binding Stability
Nguyen, Ly H.
Tran, Tuyen T.
Lien Thi Ngoc Truong
Hanh Hong Mai
Nguyen, Toan T.
BIOCHEMISTRY, 2020, 59 (13) : 1378 - 1390
[38] New redesigned zinc-finger proteins: Design strategy and its application
Negi, Shigeru
Imanishi, Miki
Matsumoto, Makoto
Sugiura, Yukio
CHEMISTRY-A EUROPEAN JOURNAL, 2008, 14 (11) : 3236 - 3249
[39] The Ros/MucR Zinc-Finger Protein Family in Bacteria: Structure and Functions
Janczarek, Monika
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (24)
[40] Crystal structure of an RNA helix recognized by a zinc-finger protein:: An 18-bp duplex at 1.6 Å resolution
Lima, S
Hildenbrand, J
Korostelev, A
Hattman, S
Li, H
RNA, 2002, 8 (07) : 924 - 932

← 1 2 3 4 5 →