Analysis of Natural Variation of the Potato Tuber Proteome Reveals Novel Candidate Genes for Tuber Bruising

被引:21
|
作者
Urbany, Claude [1 ]
Colby, Thomas [1 ]
Stich, Benjamin [1 ]
Schmidt, Lysann [1 ]
Schmidt, Juergen [1 ]
Gebhardt, Christiane [1 ]
机构
[1] Max Planck Inst Plant Breeding Res, D-50829 Cologne, Germany
关键词
potato; bruising; enzymatic discoloration; candidate genes; class III lipase; natural variation; proteomics; association genetics; POLYPHENOL-OXIDASE; ASSOCIATION GENETICS; DNA VARIATION; LATE BLIGHT; EXPRESSION; PROTEINS; RESISTANCE; IDENTIFICATION; PATATIN; FAMILY;
D O I
10.1021/pr2006186
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Potato (Solanum tuberosum) presents a challenging organism for the genetic and molecular dissection of complex traits due to its tetraploidy and high heterozygosity. One such complex trait of high agronomic interest is the tuber susceptibility to bruising upon mechanical impact, which involves an enzymatic browning reaction. We have compared the tuber proteome of two groups of 10 potato cultivars differing in bruising susceptibility to (i) identify de novo proteins that contribute to bruising, based on differential protein expression, and (ii) validate these proteins by combining proteomics with association genetics. The comparison of 20 potato varieties yields insight into the high natural variation of tuber protein patterns due to genetic background. Seven genes or gene families were found that were both differentially expressed on the protein level between groups and for which DNA polymorphisms were associated with the investigated traits. A putative class III lipase was identified as a novel factor contributing to the natural variation of bruising. Additionally, tuber proteome changes triggered by mechanical impact, within and between groups, were monitored over time. Differentially expressed proteins were found, notably lipases, patatins, and annexins, showing remarkable time-dependent protein variation.
引用
收藏
页码:703 / 716
页数:14
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