Development of an optical sensor for chlortetracycline detection based on the fluorescence quenching of L-tryptophan

A simple and selective spectrofluorimetric method for the detection of chlortetracycline (CTC) was studied. In pH 7.4 buffer medium L-tryptophan (L-Trp), applied as the fluorescence probe, interacted with CTC resulting in fluorescence quenching of the probe. CTC was detected with maximum excitation and emission wavelengths at lambda(ex)/lambda(em) = 275/350 nm. Notably, quenching of fluorescence intensities was positively proportional to the CTC concentration over the range of 0.65-30 mu mol L-1 and the limit of detection was 0.2 mu mol L-1. Effect of temperature shown in Stern-Volmer plots, absorption spectra and fluorescence lifetime determination, indicated that fluorescence quenching of L-Trp by CTC was mainly by static quenching. The proposed study used practical samples analysis satisfactorily.