Antioxidant traits and protective impact of Moringa oleifera leaf extract against diclofenac sodium-induced liver toxicity in rats

被引:38
|
作者
El-Hadary, Abdalla E. [1 ]
Ramadan, Mohamed Fawzy [2 ,3 ]
机构
[1] Banha Univ, Fac Agr, Biochem Dept, Banha, Egypt
[2] Zagazig Univ, Agr Biochem Dept, Fac Agr, Zagazig 44519, Egypt
[3] Umm Al Qura Univ, Sci Res Deanship, Mecca, Saudi Arabia
关键词
hepatoprotective; hepatotoxicity; kidney; nonsteroidal anti-inflammatory drugs; phenolic compounds; SYZYGIUM-AROMATICUM; TOTAL PHENOLICS; GLUTATHIONE; FLAVONOIDS; IBUPROFEN; LEAVES; OIL; POD;
D O I
10.1111/jfbc.12704
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Moringa oleifera gained importance as a medicinal plant. The current study assesses Moringa leaf ethanol extracts (MLE) against experimentally diclofenac sodium (DcNa)-induced liver toxicity in male rats. Leaves were extracted with different solvents differing in polarity. Assessment involved total phenolic compounds, total flavonoids and radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH center dot). HPLC was performed for identifying phenolic compounds, wherein ethyl vanillin (1,205 mg/kg), 3-OH-tyrosol (812.2 mg/kg), benzoic acid (273.8 mg/kg), salicylic acid (240.0 mg/kg), chlorogenic acid (233.3 mg/kg) and 3,4,5-methoxy-cinnamic acid (172.5 mg/kg) were measured. Fifty animals (each treatment group consisted of 10 rats) were subjected to five treatments and the experiment lasted for 4 weeks. Animals were exposed to DcNa (100 mg/kg) and two doses of MLE as well as silymarin (an antioxidant flavonoid C25H22O10) for 4 weeks. Liver marker enzymes, including alkaline phosphatase, alanine transaminase, and aspartate transaminase as well as urea, uric acid, and creatinine were increased. Serum albumin and total protein decreased in DcNa-treated rats. Homogenates nitric oxide increased in liver tissue of the DcNa-treated rats, while the activity of each of glutathione peroxidase, glutathione-S-transferase, glutathione, and catalase decreased. It could be concluded that MLE in both doses and silymarin are considerably hepatoprotective with antioxidant activity (AOA) against DcNa-induced hepatotoxicity in rats.
引用
收藏
页数:9
相关论文
共 50 条
  • [21] Protective effect of Sida cordata leaf extract against CCl4 induced acute liver toxicity in rats
    Mistry, Sunil
    Dutt, K. R.
    Jena, J.
    ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, 2013, 6 (04) : 280 - 284
  • [22] Protective effect of Sida cordata leaf extract against CCl4 induced acute liver toxicity in rats
    Sunil Mistry
    KR Dutt
    J Jena
    Asian Pacific Journal of Tropical Medicine, 2013, (04) : 280 - 284
  • [23] Moringa oleifera Leaf Extract Attenuates Pb Acetate-induced Testicular Damage in Rats
    Albasher, Gadah
    Alrajhi, Rola
    Alshammry, Entesar
    Almeer, Rafa
    COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING, 2021, 24 (10) : 1593 - 1602
  • [24] Protective effect of Moringa oleifera leaves ethanolic extract against thioacetamide-induced hepatotoxicity in rats via modulation of cellular antioxidant, apoptotic and inflammatory markers
    Mousa, Ahmed Abdelmoniem
    El-Gansh, Hala Ali Ibrahim
    Abd Eldaim, Mabrouk Attia
    Mohamed, Mostafa Abd El-Gaber
    Morsi, Azza Hassan
    El Sabagh, Hesham Saad
    ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2019, 26 (31) : 32488 - 32504
  • [25] Protective effect of Moringa oleifera leaves ethanolic extract against thioacetamide-induced hepatotoxicity in rats via modulation of cellular antioxidant, apoptotic and inflammatory markers
    Ahmed Abdelmoniem Mousa
    Hala Ali Ibrahim El-Gansh
    Mabrouk Attia Abd Eldaim
    Mostafa Abd El-Gaber Mohamed
    Azza Hassan Morsi
    Hesham Saad El Sabagh
    Environmental Science and Pollution Research, 2019, 26 : 32488 - 32504
  • [26] Chitosan/Selenium Nanoparticles Attenuate Diclofenac Sodium-Induced Testicular Toxicity in Male Rats
    El-Megharbel, Samy M.
    Al-Salmi, Fawziah A.
    Al-Harthi, Sarah
    Alsolami, Khadeejah
    Hamza, Reham Z.
    CRYSTALS, 2021, 11 (12)
  • [27] Protective role of Moringa oleifera leaf extract on cardiac antioxidant status and lipid peroxidation in streptozotocin induced diabetic rats (vol 5, e02935, 2019)
    Aju, B. Y.
    Rajalakshmi, R.
    Mini, S.
    HELIYON, 2020, 6 (01)
  • [28] The chemo-prophylactic efficacy of an ethanol Moringa oleifera leaf extract against hepatocellular carcinoma in rats
    Sadek, Kadry M.
    Abouzed, Tarek K.
    Abouelkhair, Reham
    Nasr, Sherif
    PHARMACEUTICAL BIOLOGY, 2017, 55 (01) : 1458 - 1466
  • [29] Effect of Moringa oleifera Leaf Extract on Excision Wound Infections in Rats: Antioxidant, Antimicrobial, and Gene Expression Analysis
    Al-Ghanayem, Abdullah A.
    Alhussaini, Mohammed Sanad
    Asad, Mohammed
    Joseph, Babu
    MOLECULES, 2022, 27 (14):
  • [30] Protective effects of Moringa oleifera against carbofuran induced toxicity in fish ( Labeo rohita ): Insight into hematobiochemical, histology, oxidative and antioxidant biomarkers
    Shahzadi, Muqadas
    Ahmad, Shabbir
    Rafique, Hamad
    Akmal, Hasnain
    Ditta, Allah
    Ali, Sajid
    Akram, Iqra
    Shahzad, Khurram
    KUWAIT JOURNAL OF SCIENCE, 2024, 51 (03)