Calcium-dependent hydrolysis of supported planar lipids was triggered by honey bee venom phospholipase A2 with the right orientation at the interface

被引：14
|
作者：
Kai, Siqi [1 ]
Li, Xu [1 ]
Li, Bolin [1 ]
Han, Xiaofeng [1 ]
Lu, Xiaolin [1 ]
机构：
[1] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
来源：
PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2018年 / 20卷 / 01期
基金：
中国国家自然科学基金;
关键词：
ATOMIC-FORCE MICROSCOPY; FUNCTIONAL PERSPECTIVE; COMPETITIVE-INHIBITION; STRUCTURAL-ANALYSIS; BINDING; ACTIVATION; CATALYSIS; BILAYER; SITE; SPECTROSCOPY;
D O I：
10.1039/c7cp06344j
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Hydrolysis of planar phospholipids catalyzed by honey bee venom phospholipase A(2) (bvPLA(2)) was studied. Experiments demonstrated that Ca2+ ions mediated between the lipids and bvPLA(2), induced reorientation of bvPLA(2), and activated hydrolysis. One of the hydrolysis products, fatty acids, was desorbed, and the other one, lysophospholipids, self-organized at the interface.
引用
收藏
页码：63 / 67
页数：5
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