Suppression of CSN5 promotes the apoptosis of gastric cancer cells through regulating p53-related apoptotic pathways

被引：22
|
作者：
Sang, Miao-Miao [1 ]
Du, Wen-Qi [1 ]
Zhang, Rui-Yan [1 ]
Zheng, Jun-Nian [1 ,2 ,3 ]
Pei, Dong-Sheng [1 ]
机构：
[1] Xuzhou Med Coll, Jiangsu Key Lab Biol Canc Therapy, Xuzhou 221002, Jiangsu, Peoples R China
[2] Xuzhou Med Coll, Affiliated Hosp, Ctr Clin Oncol, Xuzhou 221002, Peoples R China
[3] Xuzhou Med Coll, Jiangsu Ctr Collaborat & Innovat Canc Biotherapy, Xuzhou 221002, Jiangsu, Peoples R China
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2015年 / 25卷 / 15期
基金：
中国国家自然科学基金;
关键词：
Gastric cancer; CSN5/Jab1; siRNA; Proliferation; Apoptosis; p53; DOMAIN-BINDING PROTEIN-1; KINASE INHIBITOR P27(KIP1); COP9; SIGNALOSOME; HEPATOCELLULAR-CARCINOMA; CYTOPLASMIC LOCALIZATION; INVERSE EXPRESSION; JAB1; EXPRESSION; POOR-PROGNOSIS; ACTIVATION; DEGRADATION;
D O I：
10.1016/j.bmcl.2015.05.057
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
As one of the COP9 signalosome complex, CSN5 (also known as Jab1) has been confirmed overexpression in many human cancers and affected multiple pathways associating with cell proliferation and apoptosis. Correlation of CSN5 overexpression with poor prognosis for cancer provides evidence that it is involved in the tumorigenesis. However, little is known about the functional role and the underlying mechanism of CSN5 in gastric cancer progression. In the current study, the effect of CSN5 siRNA (small-interfering RNA) on the proliferation and apoptosis of human gastric cancer cells (AGS and MKN45) were examined. Our results showed that knockdown of CSN5 could inhibit proliferation and promote apoptosis of gastric cancer cells. Additionally, suppression of CSN5 expression contributed to the increased expression levels of p53 and Bax. In conclusion, CSN5 overexpression is significantly correlated with gastric cancer progression, and CSN5 could be a novel target in gastric cancer therapy. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2897 / 2901
页数：5
相关论文
共 50 条
  • [21] Array-based genome-wide RNAi screening to identify shRNAs that enhance p53-related apoptosis in human cancer cells
    Idogawa, Masashi
    Ohashi, Tomoko
    Sugisaka, Jun
    Sasaki, Yasushi
    Suzuki, Hiromu
    Tokino, Takashi
    [J]. ONCOTARGET, 2014, 5 (17) : 7540 - 7548
  • [22] Suppression of Jab1/CSN5 induces radio- and chemo-sensitivity in nasopharyngeal carcinoma through changes to the DNA damage and repair pathways
    Pan, Y.
    Zhang, Q.
    Atsaves, V.
    Yang, H.
    Claret, F. X.
    [J]. ONCOGENE, 2013, 32 (22) : 2756 - 2766
  • [23] Suppression of Jab1/CSN5 induces radio- and chemo-sensitivity in nasopharyngeal carcinoma through changes to the DNA damage and repair pathways
    Y Pan
    Q Zhang
    V Atsaves
    H Yang
    F X Claret
    [J]. Oncogene, 2013, 32 : 2756 - 2766
  • [24] Induction of apoptosis by the p53-related p73 and partial inhibition by the baculovirus-encoded p35 in neuronal cells
    Lo, WD
    Akhmametyeva, EM
    Zhu, LY
    Friesen, PD
    Chang, LS
    [J]. MOLECULAR BRAIN RESEARCH, 2003, 113 (1-2): : 1 - 12
  • [25] Taurine protects HK-2 cells from oxidized LDL-induced cytotoxicity via the ROS-mediated mitochondrial and p53-related apoptotic pathways
    Chang, Chun-Yu
    Shen, Chao-Yu
    Kang, Chao-Kai
    Sher, Yuh-Pyng
    Sheu, Wayne H. -H.
    Chang, Chia-Che
    Lee, Tsung-Han
    [J]. TOXICOLOGY AND APPLIED PHARMACOLOGY, 2014, 279 (03) : 351 - 363
  • [26] 13-Acetoxysarcocrassolide Induces Apoptosis on Human Gastric Carcinoma Cells Through Mitochondria-Related Apoptotic Pathways: p38/JNK Activation and PI3K/AKT Suppression
    Su, Ching-Chyuan
    Chen, Jeff Yi-Fu
    Din, Zhong-Hao
    Su, Jui-Hsin
    Yang, Zih-Yan
    Chen, Yi-Jen
    Wang, Robert Y. L.
    Wu, Yu-Jen
    [J]. MARINE DRUGS, 2014, 12 (10): : 5295 - 5315
  • [27] Verbascoside promotes apoptosis by regulating HIPK2-p53 signaling in human colorectal cancer
    Zhou, Lihong
    Feng, Yuanyuan
    Jin, Yongjie
    Liu, Xuan
    Sui, Hua
    Chai, Ni
    Chen, Xingzhu
    Liu, Ningning
    Wang, Yan
    Li, Qi
    [J]. CANCER RESEARCH, 2015, 75
  • [28] Verbascoside promotes apoptosis by regulating HIPK2-p53 signaling in human colorectal cancer
    Zhou, Lihong
    Feng, Yuanyuan
    Jin, Yongjie
    Liu, Xuan
    Sui, Hua
    Chai, Ni
    Chen, Xingzhu
    Liu, Ningning
    Ji, Qing
    Wang, Yan
    Li, Qi
    [J]. BMC CANCER, 2014, 14
  • [29] Direct contact moxibustion promotes apoptosis of gastric cancer cells in rats by regulating intestinal flora
    Lijia, P. A. N.
    Shuya, M. A.
    Jing, W. E. N.
    Xiaoqi, Zhang
    Haijiao, Xing
    Chunsheng, J. I. A.
    [J]. JOURNAL OF TRADITIONAL CHINESE MEDICINE, 2021, 41 (06) : 943 - 952
  • [30] Direct contact moxibustion promotes apoptosis of gastric cancer cells in rats by regulating intestinal flora
    PAN Lijia
    MA Shuya
    WEN Jing
    ZHANG Xiaoqi
    XING Haijiao
    JIA Chunsheng
    [J]. Journal of Traditional Chinese Medicine, 2021, 41 (06) : 943 - 952
12345