Measurement of urinary N-telopeptides from type I collagen using a colloidal gold-based immunoassay

被引：3
|
作者：
Lee, Min-Ho [2 ]
Chung, Chin Youb [1 ]
Lee, Kyoung Min [1 ]
Seong, Woo Kyeong [2 ]
Kim, Kyung Shik [3 ]
Lee, Sang Dae [3 ]
Park, Moon Seok [1 ]
机构：
[1] Seoul Natl Univ, Bundang Hosp, Dept Orthopaed Surg, Songnam 463707, Gyeonggi Do, South Korea
[2] Korea Elect Technol Inst, Songnam 463816, Gyeonggi Do, South Korea
[3] IM Elect, Hwaseong City 445170, Gyeonggi Do, South Korea
来源：
JOURNAL OF BIOTECHNOLOGY | 2011年 / 153卷 / 3-4期
关键词：
Osteoporosis; Urine N-telopeptide; Gold nanoparticle; Absorption; Bone turnover marker; BONE TURNOVER; BIOCHEMICAL MARKERS; ALENDRONATE THERAPY; OSTEOPOROSIS; RESORPTION; NANOPARTICLE; DIAGNOSIS; PUBERTY; WOMEN; SIZE;
D O I：
10.1016/j.jbiotec.2011.03.027
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
This study aimed to investigate precision of a newly developed gold nanoparticle-based immunoassay for measuring urinary N-telopeptides (NTx) and its correlation with ELISA measurement. NTx concentrations were determined by absorption changes using spectroscopy at 535 nm, which were measured three times with an interval of two hours for precision test, using urine samples of 60 subjects (32 males and 28 females; mean age 30.4 years, SD 21.9 years). The correlation of the immunoassay with the measurement of ELISA kit was also analyzed. Precision of the immunoassay and its correlation with ELISA were 0.924 (ICC, 95% confidence interval, 0.661-0.971) and 0.90 (p < 0.001). Our findings suggested that this immunoassay of urinary NTx could be used as a means of screening for bone resorption and point-of-care testing. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：176 / 180
页数：5
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