A Reproducible In-vivo Model of Lymphatic Malformation in Rats

被引：7
|
作者：
Sun, Y. [3 ,4 ,5 ,6 ]
Jia, J. [1 ,2 ]
Zhang, W. [1 ,2 ]
Liu, B. [1 ,2 ]
Zhang, Z. [3 ,4 ,5 ,6 ]
Zhao, Y. [1 ,2 ]
机构：
[1] Wuhan Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, Wuhan 430072, Hubei, Peoples R China
[2] Wuhan Univ, Hosp Stomatol, Dept Oral & Maxillofacial Surg, Wuhan 430072, Hubei, Peoples R China
[3] Wuhan Univ, Sch Stomatol, State Key Lab Breeding Base Basic Sci Stomatol, Minist Educ, Wuhan 430072, Hubei, Peoples R China
[4] Wuhan Univ, Hosp Stomatol, State Key Lab Breeding Base Basic Sci Stomatol, Minist Educ, Wuhan 430072, Hubei, Peoples R China
[5] Wuhan Univ, Hosp Stomatol, Key Lab Oral Biomed, Minist Educ, Wuhan 430072, Hubei, Peoples R China
[6] Wuhan Univ, Sch Stomatol, Key Lab Oral Biomed, Minist Educ, Wuhan 430072, Hubei, Peoples R China
来源：
JOURNAL OF COMPARATIVE PATHOLOGY | 2011年 / 145卷 / 04期
关键词：
adjuvant; lymphatic malformation; rat; VEGF-C; ENDOTHELIAL-CELLS; SAFETY; LYMPHANGIOMA; ANTIBODIES; ADJUVANTS; CHILDREN; INFANTS; D2-40; SKIN;
D O I：
10.1016/j.jcpa.2011.02.002
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
The aim of this study was to develop a reproducible rat model of lymphatic malformation. Different types of adjuvant, with and without vascular endothelial growth factor (VEGF)-C, was injected into the neck and floor of the mouth of rats. The rats were killed 2 months after the injection. Injected rats developed cystic lesions in the neck and floor of the mouth. Immunohistochemical examination revealed that the cysts were lined by endothelium; which expressed the lymphatic endothelial markers LYVE-1 and VEGF receptor-3. Raman spectra of the liquid contents of the cysts were similar in all injected rats. Transmission electron microscopy revealed that the endothelial cells had no basement membrane or surrounding pericytes. The cystic lesions were consistent with human lymphatic malformation. This animal model could be used to investigate pathogenesis of lymphatic malformation and its responses to candidate therapies. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：390 / 398
页数：9
相关论文
共 50 条
  • [1] STIMULATION OF LYMPHATIC ORGANS IN-VIVO
    SZASZ, G
    ACTA MEDICA ACADEMIAE SCIENTIARUM HUNGARICAE, 1972, 28 (3-4) : 327 - +
  • [2] In-vivo model for testing vasopressin receptor antagonists in rats
    Van Kalkeren, J
    Turnbull, Z
    Hamilton, W
    Watson, H
    Marston, HM
    JOURNAL OF PSYCHOPHARMACOLOGY, 2005, 19 (05) : A37 - A37
  • [3] AN IN-VIVO ARTERIOVENOUS MALFORMATION MODEL IN SWINE - PRELIMINARY FEASIBILITY AND NATURAL-HISTORY STUDY
    CHALOUPKA, JC
    VINUELA, F
    ROBERT, J
    DUCKWILER, GR
    AMERICAN JOURNAL OF NEURORADIOLOGY, 1994, 15 (05) : 945 - 950
  • [4] Lymphatic malformation or lymphovenous malformation
    Yavuzer, R
    Latifoglu, O
    Ataoglu, Ö
    Çenetoglu, S
    PLASTIC AND RECONSTRUCTIVE SURGERY, 1999, 104 (05) : 1579 - 1580
  • [5] IN-VIVO RADIOIMMUNOASSAY OF ALDOSTERONE IN RATS
    GLESS, KH
    HANKAPOS.M
    VECSEI, P
    ACTA ENDOCRINOLOGICA, 1973, : 156 - &
  • [6] Development of a preclinical lymphatic malformation mouse model using patient-derived lymphatic malformation progenitor cells
    Kitajewski, Christopher K.
    Wu, June K.
    Keung, Connie H.
    Reiley, Maia
    Liou, Peter
    Andrews, John
    Monteagudo, Julie
    Thirumoorthi, Arul
    Kandel, Jessica K.
    Shawber, Carrie
    ANGIOGENESIS, 2014, 17 (04) : 969 - 969
  • [7] Isolation of human lymphatic malformation endothelial cells, their in vitro characterization and in vivo survival in a mouse xenograft model
    Lokmic, Zerina
    Mitchell, Geraldine M.
    Chong, Nicholas Koh Wee
    Bastiaanse, Jacqueline
    Gerrand, Yi-Wen
    Zeng, Yiping
    Williams, Elizabeth D.
    Penington, Anthony J.
    ANGIOGENESIS, 2014, 17 (01) : 1 - 15
  • [8] Isolation of human lymphatic malformation endothelial cells, their in vitro characterization and in vivo survival in a mouse xenograft model
    Zerina Lokmic
    Geraldine M. Mitchell
    Nicholas Koh Wee Chong
    Jacqueline Bastiaanse
    Yi-Wen Gerrand
    Yiping Zeng
    Elizabeth D. Williams
    Anthony J. Penington
    Angiogenesis, 2014, 17 : 1 - 15
  • [9] CALCIUM DEFICIENCY REPLETION IN RATS AS AN IN-VIVO MODEL FOR THE STUDY OF OSTEOCLAST APOPTOSIS
    WRIGHT, KR
    MCMILLAN, PJ
    HUGHES, DE
    BOYCE, BF
    JOURNAL OF BONE AND MINERAL RESEARCH, 1995, 10 : S328 - S328
  • [10] In-vivo bioavailability and lymphatic uptake evaluation of lipid nanoparticulates of darunavir
    Bhalekar, Mangesh R.
    Upadhaya, Prashant G.
    Madgulkar, Ashwini R.
    Kshirsagar, Sanjay J.
    Dube, Akanksha
    Bartakke, Utkarsh S.
    DRUG DELIVERY, 2016, 23 (07) : 2581 - 2586
12345