Pertussis toxin increases isoproterenol induced relaxation in field-stimulated ileum

被引:7
|
作者
Sawyer, G [1 ]
Ehlert, F [1 ]
机构
[1] Univ Calif Irvine, Coll Med, Dept Pharmacol, Irvine, CA 92697 USA
关键词
acetylcholine release; pertussis toxin; muscarinic receptor; ileum; field-stimulated; muscarinic M-2 receptor;
D O I
10.1016/S0014-2999(99)00002-3
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
We investigated the effects of pertussis toxin on contraction in field-stimulated guinea pig ileum in the absence and presence of isoproterenol. Field-stimulation elicited pertussis toxin-insensitive contractions. Cumulative addition of isoproterenol produced a maximal 52% reduction in the contractile response. Following pertussis toxin-treatment, the maximal inhibitory effect of isoproterenol increased to 83%. Pertussis toxin had no effect on the ability isoproterenol to inhibit contractions elicited by histamine agonists. Our results suggest that the increased effectiveness of isoproterenol in pertussis toxin-treated ileum is due to an uncoupling of the muscarinic M-2 receptor contractile mechanism. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:81 / 84
页数:4
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