Microfluidic Impedance Flow Cytometry Enabling High-Throughput Single-Cell Electrical Property Characterization

被引：117

作者：

Chen, Jian ^{[1
]}

Xue, Chengcheng ^{[1
]}

Zhao, Yang ^{[1
]}

Chen, Deyong ^{[1
]}

Wu, Min-Hsien ^{[2
]}

Wang, Junbo ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100190, Peoples R China

[2] Chang Gung Univ, Grad Inst Biochem & Biomed Engn, Taoyuan 333, Taiwan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2015年 / 16卷 / 05期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

microfluidics; single-cell electrical property analysis; impedance flow cytometry; high throughput; RED-BLOOD-CELLS; MAXIMUM LENGTH SEQUENCES; PATCH-CLAMP TECHNIQUE; OF-CARE DIAGNOSTICS; BREAST-CANCER CELLS; MEMBRANE CAPACITANCE; CYTOPLASM CONDUCTIVITY; ON-CHIP; BIOPHYSICAL CHARACTERIZATION; PRACTICAL CONSIDERATIONS;

D O I：

10.3390/ijms16059804

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This article reviews recent developments in microfluidic impedance flow cytometry for high-throughput electrical property characterization of single cells. Four major perspectives of microfluidic impedance flow cytometry for single-cell characterization are included in this review: (1) early developments of microfluidic impedance flow cytometry for single-cell electrical property characterization; (2) microfluidic impedance flow cytometry with enhanced sensitivity; (3) microfluidic impedance and optical flow cytometry for single-cell analysis and (4) integrated point of care system based on microfluidic impedance flow cytometry. We examine the advantages and limitations of each technique and discuss future research opportunities from the perspectives of both technical innovation and clinical applications.

引用

页码：9804 / 9830

页数：27

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