Quantitative Tracking of Protein Trafficking to the Nucleus Using Cytosolic Protein Delivery by Nanoparticle-Stabilized Nanocapsules

被引：64
|
作者：
Ray, Moumita [1 ]
Tang, Rui [1 ]
Jiang, Ziwen [1 ]
Rotello, Vincent M. [1 ]
机构：
[1] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA
来源：
BIOCONJUGATE CHEMISTRY | 2015年 / 26卷 / 06期
关键词：
CELLS; IDENTIFICATION; TRANSLOCATION; LOCALIZATION; MICROSCOPY; IMPORT;
D O I：
10.1021/acs.bioconjchem.5b00141
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe a method for quantitative monitoring of subcellular protein trafficking using nanoparticle-stabilized nanocapsules for protein delivery. This method provides rapid delivery of the protein into the cytosol, eliminating complications from protein homeostasis processes found with cellularly expressed proteins. After delivery, nuclear protein trafficking was followed by real time microscopic imaging. Quantitative analyses of the accumulation percentage and the import dynamics of the nuclear protein trafficking, demonstrate the utility of this method for studying intracellular trafficking systems.
引用
收藏
页码：1004 / 1007
页数：4
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