The expression and role of stromal cell-derived factor-1α-CXCR4 axis in human dental pulp

被引：67
|
作者：
Jiang, Long [1 ]
Zhu, Ya-Qin [1 ]
Du, Rong [2 ]
Gu, Ying-Xin [2 ]
Xia, Lie [2 ]
Qin, Feng [2 ]
Ritchie, Helena H. [3 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9,Shanghai Res Inst Stomatol, Dept Gen Dent, Shanghai 200011, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9,Shanghai Res Inst Stomatol, Dept Operat Dent & Endodont, Shanghai 200011, Peoples R China
[3] Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA
来源：
JOURNAL OF ENDODONTICS | 2008年 / 34卷 / 08期
关键词：
dental pulp cells; migration; proliferation; stromal cell-derived factor-1 alpha-CXCR4 axis; tissue repair;
D O I：
10.1016/j.joen.2008.05.015
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Recent reports have suggested that the stromal cell-derived factor (SDF)-1 alpha-CXCR4 axis has a direct effect on stem and progenitor cell recruitment in muscle and neural tissue repair after injury. No information is available about SDF-1 alpha or CXC chemokine receptor 4 (CXCR4) in dental tissues. The aim of this study was to assess the expression of SDF-1 alpha and its receptor, CXCR4, in healthy or inflamed human dental pulp and to evaluate the effects of SDF-1 alpha on dental pulp cells (DPCs) in both proliferation and migration in vitro. Immunohistochemical staining and reverse-transcription polymerase chain reaction detected weak expression of SDF-1 alpha and CXCR4 in healthy dental pulp and strong expression of SDF-1 alpha and CXCR4 in inflamed dental pulp. A methylthiazol tetrazolium assay showed that SDF-1 alpha could not promote DPCs proliferation. A transmigration assay, however, indicated that SDF-1 alpha enhanced DPCs migration, which could be abolished by anti-CXCR4 antibodies. Taken together, these results imply that the SDF-1 alpha-CXCR4 axis may play a role in the recruitment of CXCR4-positive DPCs toward the damaged sites.
引用
收藏
页码：939 / 944
页数：6
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