The Synthesis and Spectral Properties of Some New Monomethine Cyanine Dyes for Live Cell Imaging

被引：0
|
作者：
Fu, Y. L. [1 ]
Dong, S. Y. [1 ]
Zhou, Y. Z. [1 ]
Zhang, G. [1 ]
Yang, Q. C. [1 ]
Zhai, X. [2 ]
Wang, L. Y. [3 ]
机构：
[1] Xian Univ Architecture & Technol, Sch Sci, Xian 710055, Peoples R China
[2] Northwest Inst Mech & Elect Engn, Xianyang 712099, Peoples R China
[3] Northwest Univ, Coll Chem & Mat Sci, Minist Educ, Key Lab Synthet & Nat Funct Mol Chem, Xian 710069, Peoples R China
来源：
BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2015年 / 36卷 / 02期
关键词：
Synthesis; Monomethine cyanine dyes; UV-Vis; Live cell imaging; FLUORESCENCE; PROBES; DNA; MICROSCOPY; DENSITY;
D O I：
10.1002/bkcs.10110
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Four monomethine cyanine dyes were synthesized and their structures were confirmed by H-1 NMR, IR, UV-Vis, and HRMS. Their spectral properties were studied. It could be found that the lambda(max) of the dyes was shorter in the protonic solvents compared with that in the non-protonic solvents. Moreover, the lambda(max) shows a hypsochromic shift with the increasing polarity of the solvents. The interactions of four dyes with DNA were also studied. The results showed that the fluorescence intensities of the dyes 3a and 3b took place a qualitative change in the presence of DNA compared with the free dyes. The dyes used as cellular imaging agents were researched. The results indicated that the dye 3b could penetrate the live cell membrane.
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页码：578 / 582
页数：5
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