Modulation of cytosolic phospholipase A2 by PPAR activators in human preadipocytes

被引:0
|
作者
Jiang, YJ [1 ]
Hatch, GM [1 ]
Mymin, D [1 ]
Dembinski, T [1 ]
Kroeger, EA [1 ]
Choy, PC [1 ]
机构
[1] Univ Manitoba, Fac Med, Lipid Res Grp, Dept Biochem & Med Genet, Winnipeg, MB R3E 0W3, Canada
关键词
cPLA(2); PPAR alpha; clofibrate; preadipocytes; arachidonic acid; SW cells; COX-2; peroxisome proliferator-activated receptor;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cytosolic phospholipase A(2) (cPLA(2)) is responsible for the release of arachidonic acid, a precursor for eicosanoid biosynthesis, from cellular phospholipids. The objective of this study is to examine the regulation of cPLA(2) by peroxisome proliferator-activated receptor (PPAR) activators in preadipocyte SW872 (SW) cells. PPAR belong to the superfamily of nuclear hormone receptors that heterodimerize with the retinoid X receptor, In this study, the presence of both PPAR alpha and PPAR gamma was confirmed in SW cells by positive identification of their mRNA in the cellular homogenate, Clofibrate, a PPAR alpha activator, caused an enhancement of ionophore A-23187-induced arachidonate release in SW cells. This increase resulted from an enhancement of cPLA(2) activity, tvhich was caused by an increase in enzyme protein. Clofibrate at lower concentrations (10-200 muM) produced increases in the mRNA levels of cPLA(2) in a dose-response manner. At higher concentrations (>400 muM), clofibrate treatment resulted in the attenuation of the cPLA(2) mRNA level and protein expression.jlr We postulate that clofibrate, acting through the PPAR alpha, caused an induction in the transcription of cPLA(2) gene, which led to an increase in the cPLA(2) protein. The observed increase in arachidonate release in SW cells appeared to be a direct result of the enhanced cPLA(2) activity.
引用
收藏
页码:716 / 724
页数:9
相关论文
共 50 条
  • [41] Nuclear membrane mechanotransduction by cytosolic phospholipase A2
    Niethammer, P.
    Shen, Z.
    Belcheva, K. T.
    Jelcic, M.
    Hui, K.
    Katikaneni, A.
    MOLECULAR BIOLOGY OF THE CELL, 2023, 34 (02) : 99 - 99
  • [42] Recent advances in the regulation of cytosolic phospholipase A2
    Gijon, MA
    Spencer, DM
    Leslie, CC
    ADVANCES IN ENZYME REGULATION, VOL 40, 2000, 40 : 255 - 268
  • [43] Cytosolic Phospholipase A2: A Drug Target in FSGS
    Erkan, Elif
    Chhaing, Richard
    Potter, Andrew
    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2021, 32 (10): : 50 - 51
  • [44] Cytosolic Phospholipase A2 and Lysophospholipids in Tumor Angiogenesis
    Linkous, Amanda G.
    Yazlovitskaya, Eugenia M.
    Hallahan, Dennis E.
    JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 2010, 102 (18): : 1398 - 1412
  • [46] Neuronal damage by secretory phospholipase A2:: modulation by cytosolic phospholipase A2, platelet-activating factor, and cyclooxygenase-2 in neuronal cells in culture
    Kolko, M
    de Turco, EBR
    Diemer, NH
    Bazan, NG
    NEUROSCIENCE LETTERS, 2003, 338 (02) : 164 - 168
  • [47] Elevated cytosolic phospholipase A2 expression and activity in human neutrophils during sepsis
    Levy, R
    Dana, R
    Hazan, I
    Levy, I
    Weber, G
    Smoliakov, R
    Pesach, I
    Riesenberg, K
    Schlaeffer, F
    BLOOD, 2000, 95 (02) : 660 - 665
  • [48] Caspase-mediated inhibition of human cytosolic phospholipase A2 during apoptosis
    Adam-Klages, S
    Schwandner, R
    Lüschen, S
    Ussat, S
    Kreder, D
    Krönke, M
    JOURNAL OF IMMUNOLOGY, 1998, 161 (10): : 5687 - 5694
  • [49] Comparative molecular field analysis of pyrrolidine inhibitors for human cytosolic phospholipase A2
    Huang, CK
    Gao, Y
    Liu, ZM
    Liu, Y
    Lai, LH
    ACTA PHYSICO-CHIMICA SINICA, 2003, 19 (01) : 79 - 81
  • [50] Structure of Human GIVD Cytosolic Phospholipase A2 Reveals Insights into Substrate Recognition
    Wang, Hui
    Klein, Michael G.
    Snell, Gyorgy
    Lane, Weston
    Zou, Hua
    Levin, Irena
    Li, Ke
    Sang, Bi-Ching
    JOURNAL OF MOLECULAR BIOLOGY, 2016, 428 (13) : 2769 - 2779