Identification of growth promotory Pseudomonas fluorescens by restriction fragment length polymorphism analysis of purified soil DNA

被引：0
|
作者：
Kumar, C [1 ]
Goel, R [1 ]
机构：
[1] Govind Ballabh Pant Univ Agr & Technol, Dept Microbiol, Pantnagar 263145, Uttar Pradesh, India
来源：
JOURNAL OF SCIENTIFIC & INDUSTRIAL RESEARCH | 1998年 / 57卷 / 10-11期
关键词：
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
DNA fingerprinting provides a useful tool for rapid and efficient identification/differentiation of bacterial strains. The fingerprint analysis of two growth promotory fluorescent pseudomonas for their environmental monitoring is reported. Genomic DNA of the strains PRS9 and GRP(3) has been isolated and subjected to RFLP analysis. Based on the size and number of fragments, Bgl II is found better in comparison to EcoRI, XbaI and Hind III. The BgI Il analysis of purified soil DNA of the strain GRP(3) and PRS9 is described. It is revealed that RFLP patterns of both these strains are distinguishable from each other as well from unsterile uninoculated control soil DNA. On the other hand DNA sample from combination trial of strains exhibit altogether different patterns.
引用
收藏
页码：575 / 578
页数：4
相关论文
共 50 条
  • [1] THE IDENTIFICATION OF CANDIDA-ALBICANS STRAINS BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF DNA
    SMITH, RA
    HITCHCOCK, CA
    EVANS, EGV
    LACEY, CJN
    ADAMS, DJ
    JOURNAL OF MEDICAL AND VETERINARY MYCOLOGY, 1989, 27 (06): : 431 - 434
  • [2] RAPID DNA PURIFICATION FOR RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS
    LINDBLOM, B
    HOLMLUND, G
    GENE ANALYSIS TECHNIQUES, 1988, 5 (05): : 97 - 101
  • [3] Identification of heterotrophic nanoflagellates by restriction fragment length polymorphism analysis of small subunit ribosomal DNA
    Lim, EL
    Dennett, MR
    Caron, DA
    JOURNAL OF EUKARYOTIC MICROBIOLOGY, 2001, 48 (03) : 247 - 257
  • [4] DNA RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM (RFLP) ANALYSIS IN LETTUCE
    LANDRY, BS
    LEUNG, H
    MICHELMORE, RW
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1986, : 24 - 24
  • [5] Identification of a novel DNA probe for strain typing Mycobacterium bovis by restriction fragment length polymorphism analysis
    O'Brien, R
    Flynn, O
    Costello, E
    O'Grady, D
    Rogers, M
    JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (05) : 1723 - 1730
  • [6] GUIDELINES FOR A QUALITY ASSURANCE PROGRAM FOR DNA RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS
    KEARNEY, JJ
    CRIME LABORATORY DIGEST, 1989, 16 (02): : 40 - 59
  • [7] RAPID IDENTIFICATION OF PSEUDOMONAS SPP. FROM AQUATIC SAMPLES USING TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS
    Luna, Gian Marco
    Danovaro, Roberto
    JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY, 2008, 16 (04): : 351 - 373
  • [8] Species identification of Streptococcus porcinus by restriction fragment length polymorphism analysis of 16S ribosomal DNA
    Abdulmawjood, A
    Weiss, R
    Lämmler, C
    RESEARCH IN VETERINARY SCIENCE, 1998, 65 (01) : 85 - 86
  • [9] Genotypic identification of Rickettsia tsutsugamushi by restriction fragment length polymorphism analysis of DNA amplified by the polymerase chain reaction
    Horinouchi, H
    Murai, K
    Okayama, A
    Nagatomo, Y
    Tachibana, N
    Tsubouchi, H
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1996, 54 (06): : 647 - 651
  • [10] IDENTIFICATION OF ROSE CULTIVARS BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM
    RAJAPAKSE, S
    HUBBARD, M
    KELLY, JW
    ABBOTT, AG
    BALLARD, RE
    SCIENTIA HORTICULTURAE, 1992, 52 (03) : 237 - 245
12345