A method for producing multiple forms of metleghemoglobin reductase and leghemoglobin components from lupine nodules

被引：4
|
作者：
Shleev, SV [1 ]
Rozov, FN [1 ]
Topunov, AF [1 ]
机构：
[1] Russian Acad Sci, Bach Inst Biochem, Moscow 117071, Russia
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2001年 / 37卷 / 02期
基金：
俄罗斯基础研究基金会;
关键词：
D O I：
10.1023/A:1002892117894
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A method for producing simultaneously homogeneous preparations of two main forms of metleghemoglobin reductase and major leghemoglobin components from lupine nodules was developed. The method involves salting our with ammonium sulfate at 40-80% saturation, gel filtration on Ultrogel AcA 44, and double isoelectric focusing. The homogeneous metleghemoglobin reductase forms with molecular weights of 62 and 66 kDa were purified 725- and 402-fold, respectively. The total yield with respect to activity equaled 37%. The 62-kDa form was more active.
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页码：195 / 200
页数：6
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