Src phosphorylation of cortactin enhances actin assembly

被引：182
|
作者：
Tehrani, Shandiz
Tomasevic, Nenad
Weed, Scott
Sakowicz, Roman
Cooper, John A.
机构：
[1] Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
[2] Cytokinet Inc, San Francisco, CA 94080 USA
[3] W Virginia Univ, Mary Babb Randolph Canc Ctr, Morgantown, WV 26506 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2007年 / 104卷 / 29期
关键词：
N-WASp; Nck; tyrosine phosphorylation;
D O I：
10.1073/pnas.0701077104
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Src kinase mediates growth factor signaling and causes oncogenic transformation, which includes dramatic changes in the actin cytoskeleton, cell shape, and motility. Cortactin was discovered as a substrate for Src. How phosphorylation of cortactin can enhance actin assembly is unknown. Here, using an actin assembly system reconstituted from purified components, we demonstrate for the first time a biochemical mechanism by which Src phosphorylation of cortactin affects actin assembly. The adaptor Nck is an important component of the system, linking phosphorylated cortactin with neuronal WASp (N-WASp) and WASp-interacting protein (WIP) to activate Arp2/3 complex.
引用
收藏
页码：11933 / 11938
页数：6
相关论文
共 50 条
  • [21] Actin depolymerization-induced tyrosine phosphorylation of cortactin: the role of Fer kinase
    Fan, LZ
    Di Ciano-Oliveira, C
    Weed, SA
    Craig, AWB
    Greer, PA
    Rotstein, OD
    Kapus, AS
    BIOCHEMICAL JOURNAL, 2004, 380 : 581 - 591
  • [22] Src- and Erk-mediated cortactin phosphorylation modulates ECM degradation at invadopodia
    Ayala, Inma
    Baldassarre, Massimiliano
    Giacchetti, Giada
    Luini, Alberto
    Buccione, Roberto
    CANCER RESEARCH, 2006, 66 (08)
  • [23] Synapsin phosphorylation by Src tyrosine kinase enhances Src activity in synaptic vesicles
    Onofri, Franco
    Messa, Mirko
    Matafora, Vittoria
    Bonanno, Giambattista
    Corradi, Anna
    Bachi, Angela
    Valtorta, Flavia
    Benfenati, Fabio
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (21) : 15754 - 15767
  • [24] PHOSPHORYLATION OF SKELETAL MYOSIN ENHANCES INTERACTION WITH ACTIN
    PEMRICK, SM
    CIRCULATION, 1978, 58 (04) : 73 - 73
  • [25] Src enhances osteogenic differentiation through phosphorylation of Osterix
    Choi, You Hee
    Han, YounHo
    Lee, Sung Ho
    Cheong, Heesun
    Chun, Kwang-Hoon
    Yeo, Chang-Yeol
    Lee, Kwang Youl
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2015, 407 (0C) : 85 - 97
  • [26] Protein Kinase D Controls Actin Polymerization and Cell Motility through Phosphorylation of Cortactin
    Eiseler, Tim
    Hausser, Angelika
    De Kimpe, Line
    Van Lint, Johan
    Pfizenmaier, Klaus
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (24) : 18672 - 18683
  • [27] Inhibition of Src Reduces Cortactin Phosphorylation and Transendothelial Migration of Acute Lymphoblastic Leukemia B cells
    Camacho, Karina Elizabeth Jimenez
    Martinez, Ramon Castellanos
    Diaz, Paola Briseno
    Sevilla, Jose Luis Flores
    Schnoor, Michael
    FASEB JOURNAL, 2019, 33
  • [28] The role of Fer kinase in the F-actin depolymerization-induced phosphorylation of cortactin
    Fan, L
    Di Ciano, C
    Craig, AW
    Greer, PA
    Kapus, A
    MOLECULAR BIOLOGY OF THE CELL, 2002, 13 : 312A - 312A
  • [29] Tyrosine phosphorylation of cortactin by the FAK-Src complex at focal adhesions regulates cell motility
    Wenqi Wang
    Yang Liu
    Kan Liao
    BMC Cell Biology, 12
  • [30] Tyrosine phosphorylation of cortactin by the FAK-Src complex at focal adhesions regulates cell motility
    Wang, Wenqi
    Liu, Yang
    Liao, Kan
    BMC CELL BIOLOGY, 2011, 12
12345