Src phosphorylation of cortactin enhances actin assembly

被引：182
|
作者：
Tehrani, Shandiz
Tomasevic, Nenad
Weed, Scott
Sakowicz, Roman
Cooper, John A.
机构：
[1] Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
[2] Cytokinet Inc, San Francisco, CA 94080 USA
[3] W Virginia Univ, Mary Babb Randolph Canc Ctr, Morgantown, WV 26506 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2007年 / 104卷 / 29期
关键词：
N-WASp; Nck; tyrosine phosphorylation;
D O I：
10.1073/pnas.0701077104
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Src kinase mediates growth factor signaling and causes oncogenic transformation, which includes dramatic changes in the actin cytoskeleton, cell shape, and motility. Cortactin was discovered as a substrate for Src. How phosphorylation of cortactin can enhance actin assembly is unknown. Here, using an actin assembly system reconstituted from purified components, we demonstrate for the first time a biochemical mechanism by which Src phosphorylation of cortactin affects actin assembly. The adaptor Nck is an important component of the system, linking phosphorylated cortactin with neuronal WASp (N-WASp) and WASp-interacting protein (WIP) to activate Arp2/3 complex.
引用
收藏
页码：11933 / 11938
页数：6
相关论文
共 50 条
  • [1] PRES 45-Cortactin phosphorylation by Src kinase enhances actin polymerization
    Tehrani, Shandiz
    Cooper, John A.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2008, 235
  • [2] Src-mediated cortactin phosphorylation regulates actin localization and injurious blebbing in acinar cells
    Singh, Vijay P.
    McNiven, Mark A.
    MOLECULAR BIOLOGY OF THE CELL, 2008, 19 (05) : 2339 - 2347
  • [3] Inducible phosphorylation of cortactin is not necessary for cortactin-mediated actin polymerisation
    Illés, A
    Enyedi, B
    Tamás, M
    Balázs, A
    Bögel, G
    Buday, L
    CELLULAR SIGNALLING, 2006, 18 (06) : 830 - 840
  • [4] Tyrosine phosphorylation of cortactin by c-Src requires Rac-mediated localization to the cortical actin cytoskeleton
    Weed, SA
    Kinley, AW
    Karginov, AV
    Head, JA
    Schaefer, EM
    Parsons, JT
    MOLECULAR BIOLOGY OF THE CELL, 2001, 12 : 286A - 287A
  • [5] The Src Substrate SKAP2 Regulates Actin Assembly by Interacting with WAVE2 and Cortactin Proteins
    Shimamura, Shintaro
    Sasaki, Kazuki
    Tanaka, Masamitsu
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2013, 288 (02) : 1171 - 1183
  • [6] PKC phosphorylation of cortactin is implicated in the regulation of actin dynamics
    Yamada, H.
    Abe, T.
    Masumoto, T.
    Sei, M.
    Kikuchi, T.
    Tomizawa, K.
    Ikeda, S.
    Matsui, H.
    Takei, K.
    MOLECULAR BIOLOGY OF THE CELL, 2011, 22
  • [7] Down-regulation of the filamentous actin cross-linking activity of cortactin by src-mediated tyrosine phosphorylation
    Huang, C
    Ni, YS
    Wang, T
    Gao, YM
    Haudenschild, CC
    Zhan, X
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (21) : 13911 - 13915
  • [8] Cortactin: coupling, membrane dynamics to cortical actin assembly
    Weed, SA
    Parsons, JT
    ONCOGENE, 2001, 20 (44) : 6418 - 6434
  • [9] Dynamin and cortactin regulate actin assembly and filament organization
    Schafer, DA
    Weed, SA
    Binns, D
    Karginov, AV
    Parsons, J
    Cooper, JA
    MOLECULAR BIOLOGY OF THE CELL, 2002, 13 : 316A - 316A
  • [10] Cortactin: coupling membrane dynamics to cortical actin assembly
    Scott A Weed
    J Thomas Parsons
    Oncogene, 2001, 20 : 6418 - 6434
12345