Study of the catalytic effect of copper(II)-protein complexes on luminol-H2O2 chemiluminescence reaction and its analytical application

被引：27

作者：

Li, ZP ^{[1
]}

Li, KA ^{[1
]}

Tong, SY ^{[1
]}

机构：

[1] Beijing Univ, Dept Chem, Beijing 100871, Peoples R China

来源：

ANALYTICAL LETTERS | 1999年 / 32卷 / 05期

基金：

中国国家自然科学基金;

关键词：

protein; copper(II); complex; chemiluminescence; luminol; peroxide;

D O I：

10.1080/00032719908542864

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Through flow injection analysis experiments, it was discovered that unsaturated complexes of Cu(ll) with proteins had a much stronger catalytic effect on the luminol-H2O2 chemiluminescence reaction than did Cu(II). Based on this, a new rapid and sensitive method for determination of some proteins in the range 0.1-20.0 mu g ml(-1) was established. The detection limits were at the 0.03-0.05 mu g ml(-1) level for bovine serum albumin (BSA), human serum albumin (HSA), human gamma-globulin (gamma-G), and egg albumin (EA). The method was used for the determination of proteins in human serum with satisfactory results.

引用

页码：901 / 913

页数：13

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