pBIN20:: An improved binary vector for Agrobacterium-mediated transformation

被引：20
|
作者：
Hennegan, KP [1 ]
Danna, KJ [1 ]
机构：
[1] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA
来源：
PLANT MOLECULAR BIOLOGY REPORTER | 1998年 / 16卷 / 02期
关键词：
Agrobacterium tumefaciens; binary vector; transformation;
D O I：
10.1023/A:1007444100898
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the construction of a binary vector for Agrobacterium tumefaciens-mediated transformation, pBIN20, which contains a superlinker region located between the left and right Ti border sequences. This vector, derived from pBI121, simplifies the cloning of plant expression cassettes and has been used in our laboratory to create lines of transgenic BY-2 tobacco cells. This new vector contains more than 20 unique restriction sites as well as the nptII selectable marker gene within the Ti-DNA borders.
引用
收藏
页码：129 / 131
页数：3
相关论文
共 50 条
  • [31] Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation
    Allan Wenck
    Mihály Czakó
    Ivan Kanevski
    László Márton
    Plant Molecular Biology, 1997, 34 : 913 - 922
  • [32] Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation
    Wenck, A
    Czako, M
    Kanevski, I
    Marton, L
    PLANT MOLECULAR BIOLOGY, 1997, 34 (06) : 913 - 922
  • [33] pBECKS - A flexible series of binary vectors for Agrobacterium-mediated plant transformation
    McCormac, AC
    Elliot, MC
    Chen, DF
    MOLECULAR BIOTECHNOLOGY, 1997, 8 (03) : 199 - 213
  • [34] Agrobacterium-mediated multiple gene transformation in rice using a single vector
    Cao, MX
    Huang, JQ
    Wei, ZM
    Yao, QH
    Wan, CZ
    Lu, JA
    JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2005, 47 (02) : 233 - 242
  • [35] Enhancing Agrobacterium-mediated plant transformation efficiency through improved ternary vector systems and auxotrophic strains
    Aliu, Ephraim
    Ji, Qing
    Wlazlo, Anna
    Grosic, Sehiza
    Azanu, Mercy K.
    Wang, Kan
    Lee, Keunsub
    FRONTIERS IN PLANT SCIENCE, 2024, 15
  • [36] Agrobacterium-mediated transformation of Solanum phureja
    Ducreux, LJM
    Morris, WL
    Taylor, MA
    Millam, S
    PLANT CELL REPORTS, 2005, 24 (01) : 10 - 14
  • [37] Agrobacterium-mediated transformation of Lilium longiflorum
    Hoshi, Y
    Kondo, M
    Kobayashi, H
    Mori, S
    Nakano, M
    Proceedings of the Ninth International Symposium on Flower Bulbs, Vols 1 and 2, 2005, (673): : 543 - 547
  • [38] Agrobacterium-mediated transformation of Vicia faba
    Petra Böttinger
    Anke Steinmetz
    Otto Schieder
    Thomas Pickardt
    Molecular Breeding, 2001, 8 : 243 - 254
  • [39] Agrobacterium-mediated genetic transformation of switchgrass
    Somleva, MN
    Tomaszewski, Z
    Conger, BV
    CROP SCIENCE, 2002, 42 (06) : 2080 - 2087
  • [40] On Agrobacterium-mediated genetic transformation of barley
    Tomasson, K
    Virumäe, K
    Truve, E
    FASEB JOURNAL, 1997, 11 (09): : A1102 - A1102
12345