Expression, purification, crystallization and preliminary X-ray analysis of cyclophilin A from the bovine parasite Trypanosoma brucei brucei

被引：3
|
作者：
Dao-Thi, MH
Transue, TR
Pelle, R
Murphy, NB
Poortmans, F
Steyaert, J
机构：
[1] Free Univ Brussels VIB, B-1640 Rhode St Genese, Belgium
[2] Int Livestock Res Inst, Nairobi, Kenya
[3] Vlaamse Instelling Technol Onderzoek, B-2400 Mol, Belgium
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1998年 / 54卷
关键词：
D O I：
10.1107/S0907444998001607
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Cyclophilin A from the bovine parasite Trypanosoma brucei brucei has been cloned, expressed in Escherichia coli, purified and crystallized in the presence of cyclosporin A using ammonium sulfate as a precipitant. The crystals belong to the orthorhombic crystal system with unit-cell dimensions of a = 118.61, b = 210.15 and c = 153.21 Angstrom. A data set complete to 2.7 Angstrom has been collected using rotating-anode radiation, however the crystals diffract to at least 2.1 Angstrom resolution using synchrotron radiation.
引用
收藏
页码：1046 / 1048
页数：3
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