Indigenous microbial diversity in conglomerate oil reservoirs analyzed by PCR-DGGE

被引:0
|
作者
She, Yuehui [1 ,2 ]
Zhang, Fan [1 ]
Shu, Fuchang [1 ]
Zhao, Liping [3 ]
Yu, Longjiang [2 ]
机构
[1] Yangtze Univ, Coll Chem & Environm Engn, Jinzhou 434023, Peoples R China
[2] Huazhong Normal Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430070, Peoples R China
[3] Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Shanghai 200240, Peoples R China
关键词
injection well; production well; denaturing gradient gel electrophoresis (DGGE); diversity; microbial enhancement of oil recovery (MEOR); POLYMERASE-CHAIN-REACTION; 16S RIBOSOMAL-RNA; NORTH-SEA; COMMUNITY STRUCTURE; FIELD; POPULATIONS; WATERS; GENES;
D O I
暂无
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Recent investigations of the microbial communities in an oil reservoir using culture-independent molecular techniques has indicated that this environment contains many uncultured assemblages, and that the cultured bacteria may represent very minor components of the true microbial diversity. In this study, 16S rDNA based PCR amplification and denaturing gradient gel electrophoresis (PCR-DGGE) was used to monitor the microbial diversity in an injection well (12#9-11) and two related production wells (12#9-9S, 13#11-8) in the KELAMAYI oilfield (Xin Jiang Autonomous Region). The DGGE gel profiles showed significant difference between the microbial community structure of injection and production wells. The bacterial species richness in the injection well was higher than those of the production ones. Unweighted Pair Group Method Clustering (UPGMC) showed the similarity between the injection well and two production wells was 30% and 20% respectively, whereas the similarity between the two production wells was 54%. In addition, the dominant bands in the DGGE gel were excised and analyzed by nucleotide sequence analysis. This analysis of 10 excised bands showed that the dominant microorganisms in all samples had high sequence identity with rRNA genes from uncultured bacteria in the GenBank database (NCBI). Nine sequences of excised band were affiliated with alpha, gamma, delta, epsilon -Proteobacteria, and one was related to Bacteroides. Two bands exhibited 100% identity to sequences of Pseudomonas stutzeri and Aminobacter sp. cox, respectively. Three bands showed relatively low identity (92% to 93%) to cultured bacteria in the GenBank database, indicating possible new taxa. This work analyzed the composition of the microbial community in KELAMAYI oilfield, which provides important data which may facilitate the isolation of new bacteria from this special underground environment. It also provides useful information for understanding the mechanism of MEOR and applying MEOR in practical oil production in KELAMAYI oilfield.
引用
收藏
页码:274 / +
页数:2
相关论文
共 50 条
  • [1] Primary study on the microbial diversity in oil polluted soils by the method of PCR-DGGE
    Wu W.
    Zhang X.
    Shan B.
    Zhang J.
    Zhao C.
    [J]. Shiyou Xuebao, Shiyou Jiagong/Acta Petrolei Sinica (Petroleum Processing Section), 2010, 26 (04): : 547 - 550
  • [2] Microbial Diversity of Ground Beef Products in South Korean Retail Market Analyzed by PCR-DGGE and 454 Pyrosequencing
    Koo, Ok-Kyung
    Kim, Hyun-Jung
    Baker, Christopher A.
    Park, Si Hong
    Ricke, Steven C.
    [J]. FOOD BIOTECHNOLOGY, 2016, 30 (01) : 63 - 77
  • [3] Monitoring exogenous and indigenous bacteria by PCR-DGGE technology during the process of microbial enhanced oil recovery
    Wang, Jun
    Ma, Ting
    Zhao, Lingxia
    Lv, Jinghua
    Li, Guoqiang
    Zhang, Hao
    Zhao, Ben
    Liang, Fenglai
    Liu, Rulin
    [J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2008, 35 (06) : 619 - 628
  • [4] Analysis of microbial diversity in Shenqu with different fermentation times by PCR-DGGE
    Liu, Tengfei
    Jia, Tianzhu
    Chen, Jiangning
    Liu, Xiaoyu
    Zhao, Minjie
    Liu, Pengpeng
    [J]. BRAZILIAN JOURNAL OF MICROBIOLOGY, 2017, 48 (02) : 246 - 250
  • [5] Analysis of microbial diversity in tomato paste wastewater through PCR-DGGE
    Sun, Shiyang
    Guo, Zhiguo
    Yang, Ruili
    Sheng, Zhigang
    Cao, Peng
    [J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2013, 18 (01) : 111 - 118
  • [6] Analysis of microbial diversity in tomato paste wastewater through PCR-DGGE
    Shiyang Sun
    Zhiguo Guo
    Ruili Yang
    Zhigang Sheng
    Peng Cao
    [J]. Biotechnology and Bioprocess Engineering, 2013, 18 : 111 - 118
  • [7] Microbial community structure of beer wastewater treatment plants analyzed by PCR-DGGE technique
    Zhao, Ji-Hong
    He, Shu-Ying
    Li, Ji-Xiang
    Liu, Yong-De
    Lou, Yan
    [J]. Huanjing Kexue/Environmental Science, 2008, 29 (10): : 2950 - 2955
  • [8] Profiling of Microbial Community Diversity in Vermifiltration of Different Earthworm abundances by PCR-DGGE
    Li, Xiaowei
    Yang, Jian
    Zhao, Limin
    Xing, Meiyan
    Deng, Dehan
    Yi, Danghao
    [J]. 2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11, 2009, : 4693 - 4695
  • [9] Assessment of the microbial diversity of Brazilian kefir grains by PCR-DGGE and pyrosequencing analysis
    Leite, A. M. O.
    Mayo, B.
    Rachid, C. T. C. C.
    Peixoto, R. S.
    Silva, J. T.
    Paschoalin, V. M. F.
    Delgado, S.
    [J]. FOOD MICROBIOLOGY, 2012, 31 (02) : 215 - 221
  • [10] Relationship of microbial diversity from recycled injection water and high-temperature petroleum reservoirs analyzed by polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE)
    Lan, Guihong
    Li, Zengting
    Zhang, Hui
    Zou, Changjun
    Qiao, Dairong
    Cao, Yi
    [J]. AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011, 10 (53): : 11004 - 11010