Analysis of microbial diversity in tomato paste wastewater through PCR-DGGE

被引:12
|
作者
Sun, Shiyang [1 ,2 ]
Guo, Zhiguo [1 ,2 ]
Yang, Ruili [1 ,2 ]
Sheng, Zhigang [3 ]
Cao, Peng [1 ,2 ]
机构
[1] Shihezi Univ, Coll Chem & Chem Engn, Shihezi 832003, Peoples R China
[2] Key Lab Green Proc Chem Engn Xinjiang Bingtuan, Shihezi 832003, Peoples R China
[3] Shihezi Tianye Tomato Prod Co LTD, Shihezi 832000, Peoples R China
关键词
wastewater treatment; PCR-DGGE; bacterial community structure; ATP; GRADIENT GEL-ELECTROPHORESIS; ACTIVATED-SLUDGE; COMMUNITY; POPULATIONS; EVOLUTION; ECOLOGY; SEARCH;
D O I
10.1007/s12257-012-0155-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This study was conducted to evaluate the relationship between the flora and the changes in the microbial communities during tomato paste wastewater treatment. The bio-analytical techniques like Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), adenosine-triphosphate (ATP) analysis, and testing of mixed liquid and suspended solids (MLSS) were simultaneously conducted to analyze the dynamics of the microbial communities during tomato paste wastewater treatment process. The study suggests that the combined approaches of PCR-DGGE, ATP, and MLSS provided a simple and accurate method to evaluate the changes in microbial activity, microbial structure, and population size with the shift in contaminants in different treatment processes. The study also demonstrates that the structure and quantity of a microbial community are influenced by MLSS during the wastewater treatment process, which consequently determines the overall functionality of the system.
引用
收藏
页码:111 / 118
页数:8
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