PREPARATION OF COMPOSITE LIPOSOMES FOR TARGETED DRUG DELIVERY

Nanotechnology is positively impacting the development of modern pharmaceutical industry. One of extensively studied drug delivery systems in clinical practice are liposomes, which have been used for enhancement of the ability to apply highly toxic drugs and overcoming of barriers to cellular and tissue uptake. The objective of the present work was to obtain the stable chitosan-coated liposomes and their interaction with anti-viral drugs. Liposomes were prepared by stepwise extrusion through 0.2 and 0.1 mu m pore sizes using inorganic membranes Anotop. Several series of experiments using the various liposomal compositions were conducted. The liposomal dispersions obtained were compared in terms of particle size, polydispersity index, zeta potential and stability. The optimal composition of the lipid film was selected and the effect of various additives was investigated. It was shown that the addition of the polyethylene glycol - modified natural monoacylglycerides and biocompatible choline derivatives of limiting fatty acids allows to reduce the diameter of the liposomes obtained by the extrusion to 150-155 nm with a polydispersity index of 0.11 - 0.13. Liposomes from the best lipid composition were prepared in a biggest quantity and coated by modified chitosan. It was concluded that appropriate combination of the liposomes and polysaccharide layer might be used for the increasing of colloidal stability up to 5 months and broad functional capabilities for surface modification. Obtained composite liposomes were loaded with selected anti-viral drugs and used as a model objects for the parenteral administration.