Amino Acids for Diels-Alder Reactions in Living Cells

被引：262

作者：

Plass, Tilman ^{[1
]}

Milles, Sigrid ^{[1
]}

Koehler, Christine ^{[1
]}

Szymanski, Jedrzej ^{[1
]}

Mueller, Rainer ^{[1
]}

Wiessler, Manfred ^{[2
]}

Schultz, Carsten ^{[1
]}

Lemke, Edward A. ^{[1
]}

机构：

[1] EMBL, Struct & Computat Biol Unit, D-69117 Heidelberg, Germany

[2] DKFZ, D-69120 Heidelberg, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 17期

关键词：

amber suppression; click chemistry; Diels-Alder reactions; protein engineering; unnatural amino acids; RECOMBINANT PROTEINS; CLICK CHEMISTRY; LIGATION; ALKYNE; DNA; CYCLOADDITIONS; STRATEGIES; AZIDES;

D O I：

10.1002/anie.201108231

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Under tension: A set of genetically encoded unnatural amino acids can be used for biocompatible site-specific labeling of proteins with fluorogenic dyes. The new compounds have norbornene and trans-cyclooctene units that react with tetrazine derivatives in an inverse-electron-demand Diels-Alder cycloaddition (left in picture). The technique offers fast labeling that is orthogonal to labeling through azide-cyclooctyne click reaction (right). Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：4166 / 4170

页数：5

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