Analyses of a Bacillus subtilis homologue of the Na+/H+ antiporter gene which is important for pH homeostasis of alkaliphilic Bacillus sp. C-125

被引：48
|
作者：
Kosono, S [1 ]
Morotomi, S [1 ]
Kitada, M [1 ]
Kudo, T [1 ]
机构：
[1] Inst Phys & Chem Res, Wako, Saitama 3510198, Japan
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1999年 / 1409卷 / 03期
关键词：
Na+/H+ antiporter; pH homeostasis; Na+ transporter; (Bacillus subtilis);
D O I：
10.1016/S0005-2728(98)00157-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bacillus subtilis was revealed to have a homologous region to the DNA fragment responsible for alkaliphily of alkaliphilic Bacillus sp. C-125 on the genome, as reported previously [1]. The yufT gene on the B. subtilis genome showed a significant similarity with ORF1 of Bacillus sp. C-125, which is related to membrane potential (Delta Psi)-driven Na+/H+ antiport activity and is important for pH homeostasis in an alkaline condition. Disruption of the yufT gene resulted in the decrease of Na+/H+ antiport activity, and the growth of the yufT disrupted strain was impaired with an increase in the external Na+ concentration. We conclude that the yufT gene encodes a Na+/H+ antiporter, which has a dominant role in the extrusion of cytotoxic Na+. (C) 1999 Elsevier Science B.V. All rights reserved.
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页码：171 / 175
页数：5
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