Novel reversed-phase high-performance liquid chromatography stationary phase with oligo(ethylene glycol) "click" to silica

被引：44
|
作者：
Guo, Zhimou [1 ]
Liu, Yanfang [1 ]
Xu, Junyan [1 ,2 ]
Xu, Qing [1 ]
Xue, Xingya [1 ]
Zhang, Feifang [1 ]
Ke, Yanxiong [1 ,2 ]
Liang, Xinmiao [1 ,2 ]
Lei, Aiwen [3 ]
机构：
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China
[2] E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
[3] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1191卷 / 1-2期
关键词：
oligo(ethylene glycol); stationary phase; click chemistry; high-performance liquid chromatography;
D O I：
10.1016/j.chroma.2007.11.093
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Oligo(ethylene glycol) (OEG) covalently bonded silica was prepared by using click chemistry and employed as a stationary phase for reversed-phase high-performance liquid chromatography. The column efficiency and effect of organic modifier content on retention were investigated. The separation selectivity was also studied with phenyl compounds and an actual sample of natural products. The results indicated that the stationary phase possessed good separation efficiency and separation selectivity in R-P-HPLC mode. Moreover, the stationary phase showed good complementary separation selectivity to the C18 stationary phase. The OEG stationary phase had "clustering" function for "homologous component" in the separation of natural products. (c) 2007 Elsevier B.V. All rights reserved.
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页码：78 / 82
页数：5
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