Radiographic, Histologic, and Biomechanical Evaluation of Combined Application of Platelet-rich Fibrin with Blood Clot in Regenerative Endodontics

被引:41
|
作者
Zhou, Ronghui [1 ,2 ]
Wang, Yumin [1 ,2 ]
Chen, Yuemin [1 ,2 ]
Chen, Shuai [1 ,2 ]
Lyu, Hongbing [1 ,2 ]
Cai, Zhiyu [3 ]
Huang, Xiaojing [1 ,2 ]
机构
[1] Fujian Med Univ, Sch & Hosp Stomatol, 246 Yangqiao Zhong Rd, Fuzhou 350002, Fujian, Peoples R China
[2] Fujian Prov Univ, Fujian Med Univ, Key Lab Stomatol, Fuzhou, Fujian, Peoples R China
[3] Fujian Med Univ, Union Hosp, Dept Stomatol, 29 Xinquan Rd, Fuzhou 350001, Fujian, Peoples R China
关键词
Blood clot; cementumlike tissue; fracture resistance; platelet-rich fibrin; regenerative endodontic treatment; IMMATURE PERMANENT TEETH; APICAL PERIODONTITIS; STEM-CELLS; REVASCULARIZATION; TOOTH; PLASMA; BONE; CONCENTRATE; EFFICACY; OUTCOMES;
D O I
10.1016/j.joen.2017.07.021
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Introduction: No in vivo study has been reported on the mechanical reinforcement of a tooth after regenerative endodontic treatment (RET). The present work aimed to evaluate the concurrent use of platelet-rich fibrin (PRF) with a blood clot (BC) in RET concerning periapical healing, root development, and tooth structural reinforcement. Methods: In our study, 24 premolars from 3 beagle dogs were assigned into control, BC, and PRF + BC groups. Periapical healing was monitored with quantitative measurements of root elongation and thickening radiographically. Tooth biomechanical integrity was assessed with the fracture resistance test. Histologic evaluation was conducted. Results: There was a significant difference among the periapical radiolucency decreasing rate of the control (43.75%) and the BC (100%) and PRF + BC (100%) groups (P < .05). The increase of root length and thickness in both the BC and PRF + BC groups was significantly greater than that in the control group (P < .05). No significant difference was detected between the 2 experimental groups regarding periapical healing or root development (P > .05). Teeth in the BC (249.3 +/- 64.1 N) and PRF + BC (281.7 +/- 37.8 N) groups had significantly higher fracture resistance than those in the control group (108.5 +/- 23.3 N) (P < .05). No significant difference was revealed between the BC and PRF + BC groups (P > .05). Histologic evidence of cementumlike tissue deposition along the canal wall with scattered bonelike tissue in the canal was observed. Conclusions: Either a combination of PRF with BC or BC alone could improve periapical healing, induce root development, and reinforce tooth structure. No additional benefit of PRF to BC in RET was found.
引用
收藏
页码:2034 / 2040
页数:7
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