An ultrasensitive electrochemical DNA sensor based on the ssDNA-assisted cascade of hybridization reaction

被引：50
|
作者：
Yin, Bin-Cheng [1 ]
Guan, Yi-Meng [1 ]
Ye, Bang-Ce [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 35期
关键词：
ROLLING CIRCLE AMPLIFICATION; SEQUENCE-SPECIFIC DETECTION; COMPLEX MATRICES; FEMTOMOLAR DNA; APTAMER; BIOSENSOR; TARGETS; PROBES; ATP; NANOPARTICLES;
D O I：
10.1039/c2cc30997a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We have developed a simple and ultrasensitive E-DNA sensor based on the ssDNA-assisted cascade of a hybridization reaction mechanism to form a long concatamers structure to improve its sensitivity, significantly. The proposed sensor was applied to sequence-specific DNA and ATP detection. Experimental results showed a quantitative measurement with the detection limit as low as 1 aM for specific DNA and 10 fM for ATP.
引用
收藏
页码：4208 / 4210
页数：3
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