The preparation and crystallization of Fab fragments of a family of mouse esterolytic catalytic antibodies and their complexes with a transition-state analogue

被引：2
|
作者：
Muranova, TA
Ruzheinikov, SN
Sedelnikova, SE
Moir, A
Partridge, LJ
Kakinuma, H
Takahashi, N
Shimazaki, K
Sun, J
Nishi, Y
Rice, DW
机构：
[1] Univ Sheffield, Krebs Inst Biomolec Res, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England
[2] Japan Tobacco Inc, Lab Life Sci & Biomol Engn, Kanagawa, Japan
[3] Russian Acad Sci, Branch Inst Bioorgan Chem, Pushchino 142292, Moscow Region, Russia
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2001年 / 57卷
关键词：
D O I：
10.1107/S0907444901010149
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The Fab fragments of a family of mouse esterolytic monoclonal antibodies MS6-12, MS6-126 and MS6-164 have been obtained by digestion of whole antibodies with papain, purified and crystallized in a range of different forms either alone or in complex with a transition-state analogue. The crystals diffract X-rays to resolutions between 2.1 and 1.2 Angstrom and are suitable for structural studies. The determination of these structures could be important in understanding the different catalytic power of each of these related catalytic antibodies.
引用
收藏
页码：1192 / 1195
页数：4
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