Cloning and characterization of c-phycocyanin operon from the cyanobacterium Arthrospira platensis FACHB341

被引:7
|
作者
Liu, JJ [1 ]
Zhang, XC [1 ]
Sui, ZH [1 ]
Zhang, XH [1 ]
Mao, YX [1 ]
机构
[1] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
关键词
Arthrospira; cpc operon; in vitro PCR; promoter sequence; Spirulina;
D O I
10.1007/s10811-005-6418-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
By using in vitro PCR method, C-phycocyanin operon of Arthrospira platensis FACHB341 was cloned and characterized. The operon consists of 427 bp ussB, 519 bp cpcB gene, 111 bp igsB-A region, 489 bp cpcA gene, 184 bp ussH region and 357 bp cpcH gene. Promoter prediction and signal scan show that there are putative promoter sequences and regulatory elements in ussB and ussH sequences.
引用
收藏
页码:181 / 185
页数:5
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