Mass spectrometry-based DNA adductomics

DNA adducts can be induced by both endogenous and external sources. Qualitative and quantitative characterizations of DNA adducts are crucial to understanding their roles in the etiology of human diseases. With the advances in mass spectrometry instrumentation, DNA adductomics aims to characterizing comprehensively DNA adducts, i.e., to screen and discover new DNA adducts, and to quantify a large number of adducts of interest. Here, we summarize the current workflow in DNA adductomics, with emphasis being placed on sample preparation and mass spectrometric methods. We also highlight recent applications of these methods, where we discuss the use of DNA adductomics methods in screening/identifying DNA adducts of biological importance and human health relevance. Future work on automated data processing software and targeted applications for adductome profiling will further advance the DNA adductomics field. (c) 2022 Elsevier B.V. All rights reserved.