Principles and Applications of Flow Cytometry and Cell Sorting in Companion Animal Medicine

被引：35

作者：

Wilkerson, Melinda J. ^{[1
]}

机构：

[1] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA

来源：

VETERINARY CLINICS OF NORTH AMERICA-SMALL ANIMAL PRACTICE | 2012年 / 42卷 / 01期

关键词：

Flow cytometry; Fluorochrome; Immunophenotyping; Cluster of differentiation antigens; Lymphocyte subset; Fluorescent activated cell sorting; Immune-mediated disorders; DIFFERENTIAL LEUKOCYTE COUNT; CANINE BONE-MARROW; DNA-PLOIDY; RETICULATED PLATELETS; LYMPHOCYTE SUBSETS; PERIPHERAL-BLOOD; ADVIA; 120; DOGS; ASSAY; ANTIBODIES;

D O I：

10.1016/j.cvsm.2011.09.012

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Flow cytometry measures multiple characteristics of single cells using light scatter properties and fluorescence properties of fluorescent probes with specificity to cellular constituents. The use in the veterinary clinical laborary has become more routine in veterinary diagnostic laboratories and institutions and reference laboratories. Common applications in small animal medicine includes quantitation of erythrocytes and leukocytes in automated hemology instruments, detection of antibodies to erythrocytes and platelets, immunophenotyping of leukocytes and lymphocytes in immunodeficiency syndromes or leukemias and lymphomas. DNA content analysis has not gained routine acceptance. Other applications including cell sorting and multiplexing are potential assays of the future.

引用

页码：53 / +

页数：20

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