Soluble Fas and soluble Fas ligand levels in patients with acute hepatic failure

Purpose: The Fas ligand (FasL)/Fas system is an apoptosis induction system that plays an important role in homeostasis and biophylaxis. We measured tumor necrosis factor alpha (TNF-alpha), soluble FasL (sFasL), and soluble Fas (sFas) in patients with acute hepatic failure to determine the relation between such failure and apoptosis. Materials and Methods: We assayed 21 blood samples from patients with acute hepatic failure and 8 from patients with sepsis but without acute hepatic failure. Serum TNF-a, sFas, and sFasL levels were determined by enzyme-linked immunosorbent assay. Results: sFasL levels were significantly higher in the patients with acute hepatic failure titan in the patients with sepsis (0.68 +/- 0.42 ng/mL vs. 0 ng/mL, P=.0001). No significant differences were observed in sFas levels between the two groups. A significant correlation was observed between TNF-alpha and sFas levels (r = 0.657, P = .0008); a negative correlation was observed between TNF-alpha and sFasL levels (r = 0.454, P = .038). Conclusions: Our results suggest that pathologic aggravation of acute hepatic failure are related to changes in the FasL/Fas system and that TNF-alpha and sFasL, in particular, may play hepatoprotective roles. Copyright (C) 2001 by WB. Sounders Company.