SCoT Molecular Markers and Genetic Diversity of Stellaria media (L.) Vill

Stellaria media (L.) Vill is a widely utilized plant for both decorative and therapeutic purposes. Despite great biochemical characterization, knowledge of genetic variability is limited, and no DNA marker have been utilized to study the genetic diversity of populations to date. The genetic diversity of 65 S. media individuals from 13 groups was investigated using ten start codon targeted (SCoT) markers in this study. SCoT was shown to be a reliable marker system for genetic study in S. media due to its high polymorphic bands (96.38%), polymorphic information content (0.34), and allele number (1.38). For our genetic analysis, we employed the SCoT molecular marker with the following goals: 1-to look into genetic variation among and within S. media, 2-to find genetic groupings within these 13 chickweed populations. 3-to gather information on the genetic makeup of date chickweed populations. The findings demonstrated a significant level of genetic heterogeneity within populations.