Novel validated spectrofluorimetric methods for the determination of taurine in energy drinks and human urine

Two sensitive, selective, economic and validated spectrofluorimetric methods were developed for the determination of taurine in energy drinks and spiked human urine. Method is based on fluorimetric determination of the amino acid through its reaction with Hantzsch reagent to form a highly fluorescent product measured at 490nm after excitation at 419nm. Method is based on the reaction of taurine with tetracyanoethylene yielding a fluorescent charge transfer complex, which was measured at (ex)/(em) of (360nm/450nm). The proposed methods were subjected to detailed validation procedures, and were statistically compared with the reference method, where the results obtained were in good agreement. Method was further applied to determine taurine in energy drinks and spiked human urine giving promising results. Moreover, the stoichiometry of the reactions was studied, and reaction mechanisms were postulated. Copyright (c) 2014 John Wiley & Sons, Ltd.